IGF1-LR3 0.1mg/vial Promotes Cell Growth, Protein Synthesis And Tissue Repair

IGF-1 LR3 0.1mg/vial core introduction

Long-acting insulin-like growth factor-1 analog | Micro-dose 0.1mg/vial

Generic name : Insulin-like growth factor-1 long chain analog

English name : Insulin-like Growth Factor-1 Long R3 (IGF-1 LR3)

Sequence : 83 amino acid single-chain polypeptide, with an N-terminal extension of 13 peptides, where the glutamic acid at position 3 is replaced by arginine.

Molecular weight : Approximately 9100 Da

Purity : ≥97.0% (HPLC)

Content : 0.1 mg/bottle (100 μg/bottle)

Appearance : Trace amount of white lyophilized powder

Storage : Freeze at -20℃, protect from light, and store in a dry place.

Usage and Storage Guide

IGF-1 LR3 Micro-form (0.1mg/vial):

IGF-1 LR3 0.1mg is a micro-dosage product designed for precision in vitro studies, particularly suitable for small-scale cell experiments, micro-screening, and preliminary dose exploration. Compared to conventional formulations, this micro-packaging minimizes storage risks after opening, ensuring fresh ingredients for each experiment. Its N-terminal extension and amino acid substitution design significantly reduce affinity for binding proteins, resulting in 2-3 times higher bioactivity than natural IGF-1 and an extended half-life, making it a highly sensitive tool for studying cell proliferation, differentiation, and metabolic regulation.

Due to its minute dosage and high activity, this specification is particularly suitable for researchers who need to conduct multiple concentration gradient tests, high-throughput screening, or studies on precious cell lines. Compared to larger products that require multiple repackaging, the 0.1mg individual packaging avoids activity loss caused by repeated freeze-thaw cycles, ensuring the consistency of experimental data. In in vitro experiments requiring precise dosage control, such as stem cell culture, neural cell research, and muscle cell differentiation, this specification offers greater operational flexibility and reagent stability.

IGF-1 LR3 micro-dosage applications:

Optimized for micro-volume, high-precision in vitro experiments, this product activates the downstream signaling pathway of the IGF-1 receptor, significantly promoting cell proliferation, protein synthesis, and inhibiting apoptosis even at low concentrations. This specification is suitable for cellular studies requiring precise dosage control and high reproducibility, especially when handling precious samples or conducting multiple parallel experiments.

This specification is widely used for: ① in vitro expansion and directed differentiation of stem cells; ② culture and functional maintenance of primary cells (such as myoblasts, neurons, and chondrocytes); ③ positive controls in high-throughput drug screening platforms; ④ addition of growth factors to micro-cell culture systems (such as 96/384-well plates); and ⑤ preliminary dose-response relationship studies of cell signaling pathway mechanisms.

Usage and Storage Methods

1. Reconstitution and preparation :

Recommended solvent :

Must be used : 0.1M sterile acetic acid solution with pH 3.5-4.5

Carrier protein : It is recommended to reconstitute with a pH 4.0 acetic acid solution containing 0.1% bovine serum albumin (BSA).

Working solution dilution : Dilute at least 100-fold with sterile PBS or complete culture medium containing 0.1% BSA.

Important Note : Do not dissolve directly in pure water, physiological saline, or neutral PBS , as this will cause irreversible aggregation and inactivation.

Standard preparation procedure :

Solvent preparation :

Acetic acid solution was treated using a 0.22 μm sterile filter.

The solvent should be preheated to 2-8°C (not room temperature).

Micro-reconstitution procedure :

100 μL of the recommended solvent to a 0.1 mg vial .

Use a micropipette to slowly inject the sample along the side of the vial, avoiding direct impact on the powder.

Place the vial on ice and rotate it horizontally very slowly for 2-3 minutes until the powder is completely dissolved.

Swirling, vibrating, or blowing are strictly prohibited.

Final concentration: 1 mg/mL (approximately 0.11 mM) acidic stock solution

Gradient dilution :

First dilution: Take 1 μL of stock solution + 999 μL of PBS containing 0.1% BSA → 1 μg/mL (approximately 110 nM) intermediate solution

Second dilution: Dilute the intermediate solution to the working concentration as needed (commonly 1-100 ng/mL).

The recommended dilution ratio is ≤100 times.

Repackaging :

The acidic stock solution was immediately dispensed into 2-5 μL/tube.

Use low-adsorption PCR tubes or silanized centrifuge tubes.

Clearly labeled: IGF-1 LR3, concentration, date, batch number

Working concentration reference :

Cell proliferation assay : 1-20 ng/mL

Differentiation induction experiment : 10-50 ng/mL

Signaling pathway studies : 0.1-10 ng/mL

Example of preparation and calculation : Target concentration 10 ng/mL, take 1 μL of 1 μg/mL intermediate solution and add it to 100 μL of culture medium.

Key points to note :

The entire process is carried out at low temperatures : the dissolution and dilution processes are performed on ice.

Acidic dissolution : Initial dissolution must be performed using a pH 4.0 acetic acid solution.

Slow dissolution : Only slow horizontal rotation is allowed for dissolution.

Single use : It is recommended to use each bottle at once after reconstitution.

Observe the solution : It should be clear and transparent after dissolution; any cloudy solution should be discarded.

To prevent adsorption : Use a buffer solution containing 0.1% BSA and low-adsorption consumables.

2. Storage conditions :

Unreconstituted lyophilized powder :

Storage temperature : -20℃, shelf life 12 months; -80℃ is recommended for storage.

Light-proof and moisture-proof : Original aluminum foil bag, sealed with desiccant inside.

Fixed position : Avoid frequent movement to prevent powder dispersion.

Reconstituted solution :

Acidic stock solution : After repackaging, store at -80℃ protected from light for ≤1 month.

Intermediate dilution : Store at -20℃ for ≤1 week

Working concentration solution : Prepare fresh and use immediately; use within 4 hours.

Freeze-thaw cycles : Repeated freeze-thaw cycles are strictly prohibited ; a maximum of one freeze-thaw cycle is allowed.

Transportation conditions :

must be used for transportation.

The packaging must include a temperature monitoring card.

Transportation time ≤ 72 hours

3. Stability control :

Physicochemical stability :

Stable in acidic environments (pH 3.5-4.5)

Temperature sensitivity: Approximately 2-3% activity loss per hour at temperatures above 4℃.

Mechanical sensitivity: Oscillation can lead to more than 50% loss of activity.

Maintenance of bioactivity :

Strictly adhere to the principle of "dissolving in acidic solutions and using in neutral solutions".

One-time dispensing, single freeze-thaw cycle

Thaw quickly before use and avoid leaving at room temperature.

Special requirements for micro-volume operations :

Use a calibrated micropipette

Pre-rinse with a solution containing 0.1% BSA before changing pipette tips.

All operations are completed within a clean bench.

Establish a negative control (containing only solvent) to exclude the influence of BSA.

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