IGF1-LR3 1mg/vial Powerful And Sustained Promotion Of Muscle Growth, Repair, And Anabolism.

IGF-1 LR3 1mg/vial core introduction

Long-acting insulin-like growth factor-1 analog | Anabolic peptide 1mg/vial

Generic name : Insulin-like growth factor-1 long chain analog

English name : Insulin-like Growth Factor-1 Long R3 (IGF-1 LR3)

Sequence : A single-chain polypeptide composed of 83 amino acids, an analog of natural IGF-1, with a 13-amino acid extension peptide added to its N-terminus, and the glutamic acid at position 3 replaced by arginine.

Molecular weight : Approximately 9100 Da

Purity : ≥97.0% (HPLC)

Content : 1mg/bottle

Appearance : White lyophilized powder

Storage : Freeze at -20℃, protect from light, and store in a dry place.

Usage and Storage Guide

IGF-1 LR3 (long-acting insulin-like growth factor-1):

IGF-1 LR3 is a modified analogue of natural IGF-1. Through N-terminal extension and single amino acid substitution, it significantly reduces the binding affinity to insulin-like growth factor-binding proteins, thereby greatly improving its bioavailability and in vivo half-life. Compared with natural IGF-1, it exhibits stronger and more sustained anabolic and mitotic activities. As a potent growth regulator, it plays a central role in cell proliferation, differentiation, survival, and protein, carbohydrate, and lipid metabolism by activating the insulin-like growth factor-1 receptor. Unlike short-acting natural peptides, its long-acting nature makes it more advantageous for applications in muscle growth, tissue repair, cell culture, and metabolic regulation research.

Due to its superior anabolic activity and significantly prolonged duration of action, IGF-1 LR3 is the preferred choice for researchers in muscle physiology, tissue engineering, cell biology, and metabolic studies. Compared to endogenous IGF-1, its binding to plasma-binding proteins is reduced, resulting in higher concentrations of the free active form and a more direct and effective action. This provides a powerful and durable standardized tool for studying muscle and bone growth, neuroprotection, cell culture expansion, and animal models and in vitro experiments related to diabetes-related metabolic disorders.

IGF-1 LR3 Applications:

It binds to and activates the insulin-like growth factor-1 receptor with high affinity, initiating downstream PI3K/Akt and MAPK signaling pathways, strongly promoting cell proliferation, differentiation, and protein synthesis, and inhibiting apoptosis. IGF-1 LR3 is a potent and long-acting anabolic factor based on the IGF-1 receptor signaling pathway, mainly used in research on muscle growth, tissue repair, cell culture, and systemic metabolic regulation.

IGF-1 LR3 is widely used in skeletal muscle hypertrophy and regeneration, osteoblast and chondrocyte research, neural cell protection and regeneration, stem cell expansion and culture, and diabetes metabolism research. Its well-defined growth-promoting and anabolistic mechanisms provide a high-quality research model for evaluating novel anabolic therapies, exploring tissue damage repair strategies, optimizing cell culture conditions, and studying growth factor signal transduction.

Usage and Storage Methods

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvent : Dilute acetic acid solution with pH 3.5-4.5 (e.g., 0.1M acetic acid)

Stability optimization : Acetic acid solution at pH 4.0 containing 0.1% bovine serum albumin (BSA) (reduces adsorption and aggregation).

Working solution dilution : Dilute with sterile PBS or cell culture medium containing 0.1% BSA.

Important Note : Do not use neutral or alkaline sterile water or PBS for direct reconstitution , as this can easily lead to protein aggregation, precipitation, and inactivation. Avoid vigorous shaking.

Standard preparation procedure :

Solvent preparation :

Use an acidic solvent (0.1M acetic acid with pH ~4.0) that has been sterile filtered through a 0.22μm filter membrane.

Allow the solvent temperature to return to room temperature (20-25℃).

Reconstitution procedure :

Add 1.0 mL of the recommended acidic solvent to a 1 mg vial.

Slowly pour the powder along the bottle wall to avoid direct impact on the powder.

Place the vial horizontally and roll it very gently back and forth in your palm (do not vortex, shake, or blow) until the powder is completely dissolved and the solution is clear.

Final concentration: 1 mg/mL (approximately 0.11 mM) of acidic stock solution.

Dispensing and dilution :

Immediately dispense into single-use doses (e.g., 10-20 μL) using low-adsorption cryovials (such as silanized tubes).

Before use, dilute the acidic stock solution at least 1000 times to the working concentration with sterile PBS or culture medium containing 0.1% BSA to neutralize the pH.

Clear labeling: Name, concentration, preparation date, batch number

Strictly avoid light during operation.

Working concentration/dosage reference :

In vitro cell experiments : Commonly used concentration range is 1 ng/mL to 100 ng/mL (approximately 0.1 nM to 11 nM).

Animal in vivo experiments : The commonly used dosage range is 10 to 200 μg/kg/day (subcutaneous injection).

Example of preparation calculation : To obtain a 10 ng/mL working solution, add 1 μL of stock solution to 100 mL of culture medium containing 0.1% BSA.

Key points to note :

Acidic dissolution : Initial resolution must be performed using an acidic solvent (pH 3.5-4.5) , which is crucial to prevent aggregation.

Dissolving method : Dissolve by gently rolling only . Any vigorous handling may cause protein denaturation.

Aseptic operation : Aseptic operation must be performed in a laminar flow hood.

Observe the solution : The reconstituted solution should be clear and colorless. Any solution showing turbidity, flocculent matter, or discoloration must be discarded.

Aliquoting and storage : Acidic stock solutions can be aliquoted and stored at -20℃ or -80℃. Diluted working solutions should be prepared and used immediately.

Avoid adsorption : Use a buffer containing a carrier protein (such as 0.1% BSA) for dilution and handling to reduce adsorption to the tube wall.

2. Storage conditions :

Unreconstituted lyophilized powder :

Long-term storage : Frozen at -20℃, shelf life 18 months

Optimal storage : -80°C (deep freeze), shelf life 24 months.

Strictly protected from light : Must be stored in the original light-proof aluminum foil bag.

Moisture protection : Keep dry, with built-in desiccant.

Reconstituted solution (acidic stock solution) :

Immediate dispensing : Dispense immediately after reconstitution.

Frozen storage : After repackaging, store at -20℃ for no more than 1 month, or at -80℃ for no more than 3 months.

Repeated freeze-thaw cycles are strictly prohibited : each dispensing tube may be frozen and thawed no more than once.

Diluted working solution :

Prepare and use immediately : The diluted neutral working solution should be used within 4 hours and must not be stored.

Transportation conditions :

Freeze-dried powder : Must be transported under dry ice (-78℃) conditions.

Packaging : Light-proof outer box + high-strength insulated box + temperature monitor.

3. Stability and Processing Standards :

Chemical and physical stability :

It is relatively stable in acidic aqueous solutions (pH 3.5-4.5).

It is highly susceptible to aggregation, precipitation, and deactivation under neutral or alkaline pH, high ionic strength, high temperature, or mechanical shear force.

Key points for maintaining bioactivity :

Strictly adhering to the principle of "acidic reconstitution and neutral use" is the key to maintaining activity.

Gentle handling and avoiding repeated freeze-thaw cycles are crucial.

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