Epithalon 10mg/vial Regulating Biological Rhythms And Slowing Down The Aging Process

Epitalon 10mg/bottle core introduction

Telomerase-activating peptide | Aging intervention tetrapeptide 10mg/bottle

Generic name : Ipitalon

English name : Epithalon (Epithalamin, Ala-Glu-Asp-Gly)

Sequence : Ala-Glu-Asp-Gly

Molecular weight : Approximately 390.3 Da

Purity : ≥99.0% (HPLC)

Content : 10mg/bottle

Appearance : White lyophilized powder

Storage : Freeze at -20℃, protect from light, and store in a dry place.

Usage and Storage Guide

Epihalon:

Ipitalon is a synthetic tetrapeptide derived from pineal gland extract, and studies have shown it to have potential telomerase activation and telomere maintenance effects. As an exploratory research tool, it is believed to play a role in the study of cellular senescence, tissue regeneration, and age-related functional decline by influencing telomere length and cell proliferation potential. Compared to other complex biologics, its structure is simple and well-defined, and it exhibits good solubility and stability under appropriate experimental conditions, providing a workable model tool for exploring molecular mechanisms related to aging and regeneration at the cellular level and in specific animal models.

Due to its potential regulatory role in cellular senescence, epitalon is chosen by researchers in many fields of basic aging biology, regenerative medicine, and cell lifespan research. Unlike some compounds with complex mechanisms of action, its short peptide nature makes it a standard experimental reagent in preliminary in vitro and animal experiments for studying cell cycle, telomere biology, and evaluating potential anti-aging interventions.

Epithalon Applications:

As an investigational peptide, it is used in specific experimental models to explore its potential effects on cell proliferation, telomerase activity, and aging-related biomarkers. It is primarily used for preliminary exploratory studies of aging mechanisms, cell lifespan, and regenerative potential.

It has been applied in cellular senescence models, telomere length and function studies, and preliminary exploratory experiments assessing age-related functional changes. Its use in specific in vitro cell culture systems (such as fibroblasts) and some animal models provides preliminary in vitro and preclinical research tools for exploring potential anti-aging intervention strategies.

Usage and Storage Methods

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvent : sterile water for injection or sterile phosphate buffer with a pH of ~7.0.

For internal injection : dilute with sterile saline.

Important note : Avoid using strong acids, strong bases, or solutions containing organic solvents. To maintain stability, it is recommended to use fresh, uncontaminated water or buffer solutions.

Standard preparation procedure :

Solvent preparation :

Use solvents that have been sterilely filtered through a 0.22 μm filter membrane.

The solvent temperature was restored to room temperature (20-25℃).

Reconstitution procedure :

Add 1.0 mL of the recommended solvent to a 10 mg vial.

Pour slowly along the bottle wall to avoid direct impact on the powder.

Gently rotate the vial for 3-5 minutes until the solution is completely clear.

Final concentration: 10 mg/mL (approximately 25.6 mM).

Dispensing and dilution :

Immediately dispense into single-use doses of 10-50 μL using low-adsorption cryovials.

Dilute to the required working concentration with sterile saline, PBS, or appropriate cell culture medium.

Clearly label: name, concentration, preparation date, batch number.

Working concentration/dosage reference :

In vitro cell experiments : The commonly used concentration range is 0.1 μg/mL to 10 μg/mL (approximately 0.26 μM to 26 μM). Preliminary experiments are required to optimize the concentration based on the specific cell type and experimental purpose.

In vivo animal studies : Commonly used dosage ranges should be referenced from specific research literature, and are usually low-dose ranges. It is strongly recommended to determine precise dosages based on preliminary experiments and animal ethics requirements.

Example of preparation calculation : If a 1 μM working solution is required, take about 0.04 μL of stock solution and add it to 1 mL of culture medium (Note: This is a micro-operation of a high-concentration stock solution, and it is recommended to perform serial dilution).

Key points to note :

Dissolve gently : Avoid swirling or violent shaking; simply rotate gently to dissolve.

Strict aseptic technique : Operations are performed in a clean bench and sterile consumables are used.

Completely avoid light : It is sensitive to light, and all steps must be carried out under light-protected conditions.

Prepare and use immediately : The stability is limited after reconstitution, so it is recommended to use it as soon as possible. After repackaging, store it under the specified conditions.

To avoid adsorption : dilution with a solution containing 0.1% bovine serum albumin or silanized low-adsorption tubes can reduce adsorption on the tube walls.

2. Storage conditions :

Unreconstituted lyophilized powder :

Long-term storage : Freeze at -20℃, shelf life 24 months.

Optimal storage : -80°C deep freeze, shelf life 36 months.

Strictly protected from light : Must be stored in the original light-proof aluminum foil bag.

Moisture protection : Keep dry; contains desiccant.

Reconstituted solution :

Use immediately : For best activity, use immediately after reconstitution.

Short-term storage : Refrigerate at 2-8℃ away from light, for no more than 24 hours.

Repackage and freeze : Store at -20℃ or -80℃ away from light for no more than 1 week.

Repeated freeze-thaw cycles are strictly prohibited : a maximum of one freeze-thaw cycle.

Transportation conditions :

Freeze-dried powder : transported by dry ice (-78℃).

Short-term shipping : 2-8℃ ice pack shipping, ensuring delivery within 48 hours.

Packaging : Light-proof aluminum foil bag + vacuum seal + foam insulated box.

3. Stability and Processing Standards :

Chemical and physical stability :

It is relatively stable in aqueous solutions with a pH of 6.0–8.0.

Avoid contact with strong acids, strong alkalis, and oxidizing agents.

It is sensitive to high temperatures and mechanical stress.

Maintenance of bioactivity :

Proper aliquoting and storage at -80°C are key to maintaining long-term activity.

Thaw and dispense the liquid slowly on ice or under refrigeration before use.

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