GHK-CU 50mg/vial It Effectively Promotes The Synthesis Of Collagen, Elastin, And Glycosaminoglycans.

GHK-Cu Copper Peptide 50mg/bottle Core Introduction

Tripeptide Copper Complex | Key Factor for Tissue Repair and Anti-Aging 50mg/bottle

Generic name : Glycylhistyllysine copper complex

Sequence : Gly-His-Lys-Cu²⁺

Molecular weight : Approximately 340.8 Da

Purity : ≥98.5% (HPLC)

Appearance : Blue lyophilized powder

Storage : Refrigerate at 2-8℃, away from light and stored in a dry place.

Usage and Storage Guide

GHK-Cu copper peptide:

GHK-Cu is a naturally occurring copper-ion tripeptide complex and a key signaling molecule in the human wound healing and anti-aging processes. Unlike synthetic compounds, it exists naturally in human blood plasma, with concentrations decreasing with age. Through the synergistic effect of copper ions, GHK-Cu exerts multiple repair and regeneration functions at the cellular and molecular levels, including stimulating collagen synthesis, promoting angiogenesis, regulating inflammatory responses, and exerting antioxidant effects. Its unique multifunctional properties make it of significant value in research on skin regeneration, wound repair, and tissue engineering.

Due to its excellent tissue repair and anti-inflammatory properties, GHK-Cu is preferred by many researchers in the fields of skin regeneration and tissue repair. It does not act through traditional hormone receptors, but rather directly promotes extracellular matrix remodeling by activating specific gene expression and signaling pathways. Its gentle repair mechanism and regenerative capacity in various tissues, including skin, hair, and nerves, make it an ideal tool molecule for studying skin aging, chronic wound healing, and cosmetic medicine.

Applications of GHK-Cu copper peptides:

It promotes collagen and elastin synthesis, enhances tissue repair and regeneration, and has significant antioxidant activity. GHK-Cu is a natural signaling peptide based on the bioactivity of copper ions, playing a key role in cell renewal and matrix remodeling.

GHK-Cu is widely used in in vitro and preclinical studies related to skin regeneration, wound healing, and anti-aging. Its direct stimulation of various cell types, including fibroblasts and keratinocytes, effectively promotes tissue repair without the high proliferative risk associated with traditional growth factors. Therefore, GHK-Cu provides a highly efficient and safe research model for exploring wound repair mechanisms, anti-aging treatment strategies, and developing tissue engineering technologies.

Usage and storage methods

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvent : sterile water for injection or physiological saline (0.9% NaCl)

Stability optimization : Sterile water containing 1% sodium hyaluronate (for topical application studies)

For cell experiments : Dissolve in sterile water and then dilute with serum-free culture medium.

Precautions : Avoid using water with high metal ion content or buffer solutions containing EDTA.

Standard preparation procedure :

Solvent preparation :

Solvents were sterile filtered through a 0.22 μm filter membrane.

Return the solvent to room temperature (20-25°C) to avoid overheating.

Reconstitution procedure :

Add 1.0-2.0 mL of the recommended solvent to a 50 mg vial.

Slowly pour the liquid along the side of the bottle, gently rotating the bottle to help it dissolve.

After dissolution, it should be a clear blue solution.

Recommended concentration: 25-50 mg/mL

Dispensing and dilution :

Immediately dispense into dark-colored or light-protected, low-absorption centrifuge tubes.

Dilute to the working concentration with the appropriate buffer solution according to experimental requirements.

Clear labeling: Product name, concentration, preparation date

Working concentration reference :

In vitro cell experiments : The commonly used working concentration range is 1 nM to 10 μM

Topical application studies : 0.1% to 1.5% solutions

Animal studies : The commonly used dosage range is 0.1 to 2 mg/kg/day

Example of preparation calculation : If a 1mM working solution is required, take about 3.4μL of 50mg/mL stock solution and add it to 1mL of culture medium (molecular weight about 341g/mol).

Key points to note :

Light sensitive : GHK-Cu is light sensitive, and all operations must be performed in strict light-proof conditions.

Metal contamination : Avoid contact with metal instruments and prioritize the use of plastic consumables.

Solubility : Solubles well in sterile water, forming a clear blue solution. If dissolution is difficult, gently vortex.

Aseptic operation : Strictly performed in a laminar flow hood.

Prepare and use immediately : The stability of the solution is limited after reconstitution, so it is recommended to use it as soon as possible.

2. Storage conditions :

Unreconstituted lyophilized powder :

Short-term storage : Refrigerate at 2-8℃, shelf life 12 months.

Long-term storage : Freeze at -20℃, shelf life 24 months.

Strictly protected from light : Must be stored in the original light-proof aluminum foil bag.

Moisture protection : Keep dry; contains desiccant.

Reconstituted solution :

Use immediately : For best activity, use immediately after reconstitution.

Short-term storage : Store at 2-8℃ away from light for no more than 24 hours.

Repackage and freeze : Store at -20℃ away from light for no more than 7 days.

Repeated freeze-thaw cycles are strictly prohibited : a maximum of 1-2 freeze-thaw cycles are allowed.

Transportation conditions :

Refrigerated transport : Transport with ice packs at 2-8℃.

Freeze-dried powder transportation : It can be transported at room temperature, but refrigeration is recommended to maintain optimal stability.

Packaging : Light-proof aluminum foil bag + vacuum seal + foam insulated box.

3. Stability and Processing Standards :

Chemical stability :

It is most stable in aqueous solutions with a pH of 6.0-7.5.

Strong acids, strong bases, or strong oxidizing agents can destroy its structure.

It is sensitive to certain metal ions and may undergo ligand exchange.

Physical and optical stability :

The solution is sensitive to light (especially ultraviolet light), and its color may lighten.

Avoid high temperatures, violent vibrations, and ultrasonic treatments.

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