Description

Technical Parameters

GDF-8/Myostatin 1mg/bottle Core Introduction

Pharmaceutical-grade recombinant protein | China-made growth differentiation factor 8 (GDF-8/Myostatin )

Product Name : Recombinant Human Growth Differentiation Factor 8 (Myostatin)

UniProt ID : O14793

Specification : 1mg/bottle (lyophilized powder)

Dosage form : lyophilized protein raw material

Molecular weight : Approximately 25 kDa (under reduction conditions)

Homodimer : Approximately 50 kDa (non-reducing conditions)

Purity : ≥95% (SDS-PAGE test)

Bioactivity : Verified by C2C12 cell proliferation inhibition assay

Storage temperature : -20℃ or -80℃ for long-term storage, 2-8℃ for short-term storage.

Transportation conditions : Dry ice transportation

Solubility recommendation : Sterile PBS (pH 7.2-7.4) or buffer containing 0.1% BSA.

Core Effects

GDF-8 is a member of the transforming growth factor-β (TGF-β) superfamily . As a negative regulator of muscle growth , it has important scientific research value in muscle development regulation, muscle atrophy research, and the development of treatments for muscle diseases .

✅Biological mechanism :

Muscle growth inhibition : Inhibits myoblast proliferation and differentiation through the SMAD signaling pathway.

Receptor binding : Activates the ActRIIB receptor, initiating downstream signaling cascades.

Tissue specificity : Primarily expressed and functioning in skeletal muscle.

✅Quality advantages of Chinese manufacturing :

Mammalian cell expression : HEK293 or CHO cell expression system, ensuring proper folding and glycosylation.

Affinity purification : Ni-NTA or antibody affinity chromatography, a two-step purification process.

Endotoxin control : <1.0 EU/μg, meeting the standards for injectable protein.

✅Quality Analysis Standards :

SDS-PAGE purity : ≥95% (Coomassie Brilliant Blue staining)

SEC-HPLC : Monomer content ≥90%

Western Blot : Specific antibody recognition confirmation

Mass spectrometry analysis : MALDI-TOF confirms molecular weight

Peptide mapping analysis : LC-MS/MS verification of amino acid sequences

Product Features and Advantages

1. Scientific research application value :

Muscle biology research : Research on the mechanisms of muscle development and regeneration

Disease Model Establishment : Tools for Establishing a Muscular Atrophy Model

Drug screening platform : Screening of myostatin inhibitors

Signaling pathway research : TGF-β/SMAD signaling pathway research

2. Technical parameters :

Expression system : mammalian cell expression, proper folding

Glycosylation : Natural glycosylation modification with activity consistent with endogenous proteins.

Buffer system : 20 mM Tris-HCl, 150 mM NaCl, pH 8.0

Protein concentration : ≥0.5 mg/ml before lyophilization

3. Advantages of Chinese manufacturing :

Cost advantage : Large-scale production reduces research and development costs

Advanced technology : Mature mammalian cell expression technology

Customization services : We can provide customized products with different labels, mutants, etc.

Technical Support : A professional technical team provides application support.

Usage and Storage Guide

1. Storage conditions :

Unopened : Can be stored for 12 months at -20℃, and for 24 months at -80℃.

Reconstituted : Can be stored for 7 days at 2-8℃, or for 1 month at -20℃.

Avoid repeated freeze-thaw cycles : It is recommended to store in smaller portions and avoid more than 3 freeze-thaw cycles.

Transportation conditions : Dry ice transportation, ensuring the temperature remains below -60℃ throughout the entire journey.

2. Reconstitution method :

Preparation : Remove from the refrigerator and allow to equilibrate to room temperature for 10 minutes.

Diluent selection : Sterile PBS (pH 7.2-7.4) is recommended.

Reconstitution concentration : It is recommended to reconstitute to 0.1-1.0 mg/ml.

Dissolving steps : Slowly add the buffer solution and gently vortex to mix.

Concentration determination : Protein concentration was determined using the BCA method or A280 method.

3. Recommendations for use in cell experiments :

Working concentration : Recommended concentration for cell experiments is 1-100 ng/ml

Treatment time : 24-72 hours for cell treatment

Positive control : It is recommended to use TGF-β1 as a positive control.

Negative control : Set up a serum-free culture medium control.

4. Recommendations for use in animal experiments :

Dosage : Recommended dose for mice is 1-10 mg/kg

Route of administration : intraperitoneal injection or intramuscular injection

Experimental period : The dosing time is designed according to the research objectives.

Sample collection : Collect tissue samples 24-72 hours after processing.

5. Precautions :

Aseptic technique : All procedures should be performed in a laminar flow hood.

Avoid contamination : Use sterile pipette tips and centrifuge tubes.

Avoid shaking : Avoid vigorous shaking during the dissolution process.

Prepare and use immediately : It is recommended to prepare the working solution on the day of the experiment.

Research and application directions

1. Basic research field :

Research on the molecular mechanisms of muscle development

Muscle stem cell biology research

Exploring the regulatory mechanisms of muscle regeneration

2. Disease research field :

Research on the pathogenesis of muscular dystrophy

Study on cachexia-related muscle wasting

Age-related sarcopenia research

3. Drug development research :

Screening for myostatin inhibitors

Antibody drug development

Gene therapy research

Why choose the Chinese-made GDF-8 ?

High purity : SDS-PAGE purity ≥95%, ensuring the reliability of experimental results.

High activity : Expressed in mammalian cells, ensuring proper folding and glycosylation.

Stable quality : High batch-to-batch consistency ensures experimental repeatability.

Professional Support : Provides complete technical documentation and application guides

Summary

This product is a high-purity recombinant human GDF-8/myostatin protein , manufactured using advanced mammalian cell expression technology and a rigorous quality control system in China . It provides a high-quality, highly active research tool for muscle biology and muscle disease research . With proper storage and use , researchers can obtain reliable and reproducible experimental data , advancing basic research and drug development for muscle-related diseases.

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