Description

Technical Parameters

ACE-031 Recombinant Fusion Protein 1mg/vial: Key Introduction

Pharmaceutical-grade biologic | Made in China ACE-031 (ActRIIB-Fc fusion protein)

Product Name : Recombinant Human ActRIIB-Fc Fusion Protein

Molecular structure : Human ActRIIB extracellular region + Human IgG1 Fc region

Specification : 1mg/bottle (lyophilized powder)

Dosage form : Lyophilized protein preparation for injection

Molecular weight : Approximately 120 kDa (SDS-PAGE under non-reducing conditions)

Subunit structure : homodimer

Purity : ≥95% (reduced SDS-PAGE, Coomassie brilliant blue staining)

Endotoxin : <1.0 EU/mg

Bioactivity : Verified by myostatin binding assay

Storage temperature : -80℃ for long-term storage, -20℃ for short-term storage

Storage after reconstitution : Refrigerate at 2-8℃, avoid repeated freeze-thaw cycles.

Transportation conditions : Dry ice transportation

Core Function | Core Effects

ACE-031 is a fusion protein of the extracellular region of the recombinant ActRIIB receptor and IgG1 Fc . As an antagonist of the myostatin signaling pathway , it has important scientific research value in the study of muscle atrophy treatment, muscle growth promotion and metabolic diseases .

✅Molecular mechanism of action :

Receptor binding : As a decoy receptor, it binds to TGF-β family ligands such as myostatin and activin.

Signal blocking : Blocking the binding of ligands to the ActRIIB receptor on the cell surface inhibits the SMAD signaling pathway.

Muscle growth promotion : Relieves the inhibitory effect of myostatin on muscle growth.

✅Quality advantages of Chinese manufacturing :

Mammalian expression system : CHO cell expression, ensuring proper glycosylation and folding.

Protein A purification : Protein A affinity chromatography for high-purity separation.

Quality control system : Recombinant protein quality control standards conforming to ICH Q6B guidelines.

✅Quality Analysis Standards :

SEC-HPLC purity : monomer content ≥90%, aggregates <5%

SDS-PAGE analysis : Single bands under both reducing and non-reducing conditions

Western Blot : Dual Validation with Anti-human IgG and Anti-ActRIIB Antibodies

Peptide mapping analysis : LC-MS/MS verification of amino acid sequence integrity

Glycosylation analysis : N-glycosylation site analysis

Product Features and Advantages

1. Scientific research application value :

Muscle disease research : Mechanisms of Duchenne muscular dystrophy and muscular atrophy

Muscle growth regulation : A study on the function of the myostatin signaling pathway

Metabolic Research : Study on Muscle-Fat Metabolic Interactions

Drug Development : Preclinical Research on Drugs for the Treatment of Muscle Atrophy

2. Technical parameters :

Expression vector : Mammalian expression vector containing signal peptide and fusion tag

Buffer system : 20 mM phosphate buffer, 150 mM NaCl, pH 7.4

Freeze-drying protectant : Contains 5% trehalose

Protein concentration : ≥2 mg/ml before lyophilization

3. Advantages of Chinese manufacturing :

Large-scale production : Mature CHO cell suspension culture technology

Cost advantage : It has a significant price advantage compared to imported products.

Customized services : We can provide fusion proteins with different mutants and different Fc regions.

Complete technical documentation : Provides complete COA, MSDS, and analysis certificates.

Usage and Storage Guide

1. Storage conditions :

Unopened freeze-dried powder : can be stored for 24 months at -80℃, and for 6 months at -20℃.

Reconstituted solution : can be stored at 2-8℃ for 7 days; avoid freezing.

Repackaging Recommendation : For long-term storage, it is recommended to repackage and freeze at -80℃.

Avoid repeated freeze-thaw cycles : Do not freeze-thaw more than twice to prevent protein aggregation.

2. Reconstitution method :

Preparation : After removing from -80℃, allow to equilibrate to room temperature for 10-15 minutes.

Reconstitution buffer : Sterile PBS (pH 7.2-7.4) is recommended.

Reconstitution concentration : It is recommended to reconstitute to 1-2 mg/ml

Dissolution procedure : Slowly add buffer solution along the tube wall and gently rotate to dissolve.

Concentration determination : Measured using the BCA method or A280 (extinction coefficient 1.4).

3. Recommendations for use in cell experiments :

Working concentration range : 0.1-10 μg/ml

Treatment time : 24-72 hours for cell treatment

Negative control : Isotype control IgG

Detection method : Western blot detection of p-SMAD2/3 level changes

4. Recommendations for use in animal experiments :

Dosage for mice : 1-10 mg/kg body weight

Dosage frequency : 1-2 times per week via subcutaneous injection

Experimental period : usually 2-4 weeks

Observation indicators : body weight change, muscle mass, muscle fiber cross-sectional area

5. In vitro binding assay :

ELISA binding assay : Coating with myostatin, detecting ACE-031 binding.

SPR/BLI analysis : Determination of binding kinetic parameters with ligands

Competitive binding experiment : Competitive binding with soluble ActRIIB

Research and application directions

1. Disease mechanism research :

Establishment of an animal model of muscular dystrophy

Mechanisms of cachexia-related muscle wasting

Age-related sarcopenia research

2. Signaling pathway research :

TGF-β superfamily signal transduction mechanism

Muscle-specific signaling regulatory network

Muscle regeneration and repair mechanisms

3. Translational medicine research :

Evaluation of the efficacy of drugs for the treatment of muscle atrophy

Biomarker discovery and validation

Preclinical pharmacodynamic studies

Why choose the Chinese-made ACE-031?

High purity : SEC-HPLC purity ≥90%, ensuring experimental accuracy.

High activity : Expressed in mammalian cells, maintaining native conformation and function

Stable quality : Strict production process control ensures high batch consistency.

Full support : Provides detailed technical documentation and experimental plans.

Summarize

This product is a high-purity recombinant ACE-031 fusion protein , produced using advanced mammalian cell expression and protein purification technology in China . It provides a high-quality, highly active research tool for muscle disease research and the exploration of muscle growth regulation mechanisms . Proper storage, reconstitution, and usage methods are crucial to ensuring the reliability and reproducibility of experimental results.

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