Oxytocin 2mg/vial Powerful Promotion Of Uterine Contraction And Milk Ejection

Oxytocin 2mg/vial core introduction

Neuropituitary nonapeptide hormone | Cyclic neuropeptide 2mg/bottle

Generic name : Oxytocin

English name : Oxytocin

Sequence : Cys¹-Tyr²-Ile³-Gln⁴-Asn⁵-Cys⁶-Pro⁷-Leu⁸-Gly⁹-NH₂ (disulfide bonds link Cys¹ and Cys⁶)

Molecular weight : Approximately 1007.2 Da

Purity : ≥98.0% (HPLC)

Content : 2mg/bottle

Appearance : White lyophilized powder

Storage : Freeze at -20℃, protect from light, and store in a dry place.

Usage and Storage Guide

Oxytocin:

Oxytocin is a cyclic nonapeptide hormone synthesized by the hypothalamus and released by the neurohypophysis, containing a key intramolecular disulfide bond in its structure. As a classic neuropeptide, it plays a crucial role in regulating uterine smooth muscle contraction, mammary myoepithelial cell contraction, and various social behaviors in mammals by activating specific G protein-coupled receptors. Unlike most linear peptides, its cyclic structure endows it with unique stability, making it an irreplaceable research tool for studying neuroendocrine regulation, reproductive physiology, and the neural basis of social behavior.

Due to its well-defined effects on uterine contraction and milk production, as well as its crucial role in regulating social behavior, stress responses, and mother-infant bonding, oxytocin is a preferred choice for researchers in neuroscience, reproductive biology, and behavioral science. Unlike broad-spectrum hormones, its effects are relatively specific, providing a precise tool for studying specific neural pathways and receptor functions. Its influence on maternal and infant behavior, social cognition, and stress regulation makes it a standardized and highly specific research tool in animal models studying the neural mechanisms of social behavior, stress response regulation, and reproductive-related neural pathways.

Applications of oxytocin:

Oxytocin binds to oxytocin receptors in specific regions of the uterus, breast, and brain, inducing smooth muscle contraction and regulating social behavior, stress responses, and emotional connections. Oxytocin is a classic research tool based on neuropeptide signaling pathways, primarily used to explore reproductive physiology, social neuroscience, and behavioral endocrinology.

Oxytocin is widely used in reproductive biology, neuroscience, social behavior, and stress research. Its well-defined receptor mechanism of action provides high-quality research models for studying the molecular basis of uterine contractions, exploring neural circuits involved in social behavior and emotional regulation, and assessing the role of neuropeptides in stress adaptation. It is also widely used in research exploring the neurobiological basis of mother-infant bonding, social trust, anxiety behavior, and related mental illnesses.

Usage and Storage Methods

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvent : sterile water for injection or dilute acetic acid solution (0.25% v/v acetic acid) with pH 3.5-4.5.

Stability optimization : a sterile aqueous solution containing 0.9% sodium chloride and 0.25% acetic acid.

For in vivo injection : Dilute with sterile physiological saline or the above-mentioned acidic solution.

Important Note : Neutral or alkaline solvents (such as regular PBS) must not be used, as they will rapidly oxidize and deactivate the PBS. Avoid using solvents containing metal ions or oxidizing agents.

Standard preparation procedure :

Solvent preparation :

An acidic solvent that has been sterilely filtered through a 0.22 μm filter membrane and pre-deoxygenated is used.

Allow the solvent temperature to return to room temperature (20-25℃).

Reconstitution procedure :

Add 1.0 mL of the recommended acidic solvent to a 2 mg vial.

Pour slowly along the bottle wall, avoiding vigorous agitation that could introduce air bubbles and oxygen.

Gently rotate the vial until completely dissolved, avoiding vortexing.

Final concentration: 2 mg/mL (approximately 1.99 mM)

Dispensing and dilution :

Under inert gas (such as nitrogen) protection, immediately dispense into single-use volumes (e.g., 20-50 μL) using low-adsorption cryovials.

Dilute to working concentration with pre-cooled sterile acidic saline (pH ~4.0).

Clear labeling: Name, concentration, pH, preparation date, batch number

Strictly avoid light and oxygen throughout the entire process.

Working concentration/dosage reference :

In vitro cell experiments : Commonly used concentration range is 1 nM to 1 μM

Animal in vivo experiments (central nervous system) : The usual dosage range is 0.1 to 10 μg/animal (injection into the ventricle or a specific brain region).

Animal in vivo experiments (peripheral) : The usual dose range is 1 to 100 IU/kg (intravenous or intraperitoneal injection, 1 mg ≈ 500 IU).

Example of preparation calculation : If a 100 nM working solution is required, take 0.05 μL of stock solution and add it to 1 mL of culture medium (pH neutralization needs to be considered).

Key points to note :

Acidic environment : It must be dissolved and stored in an acidic environment (pH 3.5-4.5) to maintain the stability of disulfide bonds and prevent oxidation.

Strict oxygen avoidance : Operations should be carried out under an inert atmosphere as much as possible to reduce oxidation.

Dissolve gently : Avoid vortexing, violent shaking or repeated blowing to prevent peptide chain breakage and oxidation.

Strict aseptic technique : Operations are performed in a clean bench and sterile consumables are used.

Completely avoid light : It is sensitive to light, and all steps must be carried out under light-protected conditions.

Prepare and use immediately : Under acidic conditions, it is best to store at 2-8℃ for no more than 24 hours after reconstitution.

2. Storage conditions :

Unreconstituted lyophilized powder :

Long-term storage : Frozen at -20℃, shelf life 24 months

Optimal storage : -80°C deep freeze, vacuum or nitrogen-sealed, shelf life 36 months.

Strictly protected from light : Must be stored in the original, light-proof, nitrogen-filled, airtight container.

Moisture protection : Keep dry, with built-in desiccant.

Reconstituted solution :

Use immediately : For best activity, use immediately after reconstitution.

Short-term storage : Must be prepared with an acidic solvent (pH ~4.0) , aliquoted, and stored frozen at -80°C protected from light for no more than 1 month. Storage in alkaline or neutral solutions is strictly prohibited .

Repeated freeze-thaw cycles are strictly prohibited : a maximum of one freeze-thaw cycle will result in significant loss of activity.

Transportation conditions :

Freeze-dried powder : Transported with dry ice (-78℃), preferably in vacuum nitrogen-filled packaging.

Packaging : Light-proof aluminum foil bag + vacuum/nitrogen-filled seal + foam insulated box.

3. Stability and Processing Standards :

Chemical and physical stability :

It is stable only under acidic conditions (pH 3.5-4.5) and rapidly deactivated under neutral or alkaline conditions.

It is extremely sensitive to oxidation, especially at the disulfide bond sites.

Avoid contact with metal ions, oxidants, and reducing agents.

Maintenance of bioactivity :

Maintaining an acidic environment is the most critical factor in preserving activity .

Dispensing, nitrogen purging, and storage at -80°C are standard operating procedures for maintaining long-term activity.

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