Aicar 50mg/vial Significantly Improves Aerobic Metabolic Capacity And Endurance

AICAR 50mg/bottle core introduction

AMPK activator | Adenosine analogue 50mg/vial

Generic name : 5-aminoimidazole-4-formamide ribonucleoside

English name : 5-Aminoimidazole-4-carboxamide ribonucleoside (AICAR)

CAS No .: 2627-69-2

Molecular formula : C₉H₁₅N₄O₅

Molecular weight : 259.24 g/mol

Appearance : White to off-white lyophilized powder

Content : 50mg/bottle

Storage : Freeze at -20℃, protect from light, and store in a dry place.

Usage and Storage Guide

AICAR (5-aminoimidazolium-4-carboxamide ribonucleoside):

AICAR is an adenosine analog that can be phosphorylated intracellularly by adenosine kinase to generate ZMP (5-aminoimidazole-4-carboxamide ribonucleotide). ZMP, as an AMP analog, can activate AMP-activated protein kinase (AMPK). AMPK is a key regulator of cellular energy metabolism, activated under energy deficiency conditions, promoting glucose uptake, fatty acid oxidation, and mitochondrial biosynthesis while inhibiting protein and lipid synthesis. By mimicking energy deficiency, AICAR provides an important pharmacological tool for studying the AMPK signaling pathway, energy metabolism regulation, and related disease mechanisms.

Due to its well-defined AMPK activating effect, AICAR is widely used in metabolic disease research, exercise physiology, and the exploration of cardiovascular protective mechanisms. Compared to other AMPK activators, its mechanism of action is relatively specific, activating AMPK by mimicking changes in endogenous AMP levels. This makes it a standardized and predictable key tool in animal models for studying energy balance, improved insulin sensitivity, and protection against ischemia-reperfusion injury.

AICAR Applications:

Intracellular phosphorylation generates ZMP, which activates the AMPK signaling pathway, promoting glucose uptake, fatty acid oxidation, and mitochondrial function while inhibiting anabolism. AICAR is a metabolic regulator based on the AMPK activation mechanism, primarily used to study energy metabolism, insulin sensitivity, and exercise adaptation.

AICAR is widely used in research on metabolic syndrome, type 2 diabetes, obesity, cardiovascular disease, and exercise physiology. Its well-defined pharmacological mechanism of action provides a high-quality research model for evaluating novel metabolic regulators, studying the role of AMPK in metabolic diseases, and exploring the impact of exercise training on energy metabolism.

Usage and Storage Methods

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvent : sterile water for injection or sterile PBS buffer (pH 7.0-7.4)

For internal injection : dilute with sterile saline.

Important note : Avoid using strong acids, strong alkalis, or solutions containing organic solvents.

Standard preparation procedure :

Solvent preparation :

Solvents were sterile filtered through a 0.22 μm filter membrane.

The solvent temperature was restored to room temperature (20-25℃).

Reconstitution procedure :

Add 1.0 mL of the recommended solvent to a 50 mg vial.

Slowly pour the powder along the bottle wall to avoid direct impact on the powder.

Gently rotate the vial for 3-5 minutes until completely dissolved.

Final concentration: 50 mg/mL (approximately 193 mM)

Dispensing and dilution :

Immediately dispense into single-use doses using low-absorption cryovials.

Dilute to the required working concentration with sterile saline or PBS.

Clear labeling: Name, concentration, preparation date, batch number

Strictly avoid light during operation.

Working concentration/dosage reference :

In vitro cell experiments : Commonly used concentration range is 0.1 to 1 mM

Animal in vivo experiments : The commonly used dosage range is 50 to 500 mg/kg (intraperitoneal or intravenous injection).

Example of preparation calculation : If a 0.5 mM working solution is required, take 2.6 μL of stock solution and add it to 1 mL of culture medium.

Key points to note :

Dissolve gently : Avoid swirling or violent shaking; gently rotate to dissolve.

Strict aseptic technique : Operations are performed within a laminar flow hood, using sterile consumables.

Complete protection from light : Sensitive to light, all steps must be performed under light-protected conditions.

Prepare and use immediately : Stability is limited after reconstitution, so it is recommended to use as soon as possible.

Avoid repeated freeze-thaw cycles : Avoid multiple freeze-thaw cycles after repackaging.

2. Storage conditions :

Unreconstituted lyophilized powder :

Long-term storage : Frozen at -20℃, shelf life 24 months

Optimal storage : -80°C (deep freeze), shelf life 36 months.

Strictly protected from light : Must be stored in the original light-proof aluminum foil bag.

Moisture protection : Keep dry, with built-in desiccant.

Reconstituted solution :

immediately after reconstitution for optimal activity.

Short-term storage : Refrigerate at 2-8℃ away from light, for no more than 24 hours.

Repackage and freeze : Store at -20℃ or -80℃ away from light, for no more than 1 week.

Repeated freeze-thaw cycles are strictly prohibited : a maximum of one freeze-thaw cycle.

Transportation conditions :

Freeze-dried powder : transported with dry ice (-78℃)

Short-term shipping : Transported with ice packs at 2-8℃, ensuring delivery within 48 hours.

Packaging : Light-proof aluminum foil bag + vacuum seal + foam insulated box

3. Stability and Processing Standards :

Chemical and physical stability :

It is relatively stable in aqueous solutions with a pH of 7.0–7.4.

Avoid contact with strong acids, strong alkalis, and oxidizing agents.

Sensitive to high temperatures, prolonged exposure to room temperature should be avoided.

Maintenance of bioactivity :

Proper aliquoting and storage at -80°C are key to maintaining long-term activity.

Thaw and dispense the solution slowly on ice before use.

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