Kisspeptin 10mg/vial Optimize Reproductive Endocrine Function

Kisspeptin-10 10mg/vial core introduction

Gonadotropin-releasing hormone upstream regulatory peptide | Key signaling peptide of reproductive neuroendocrine system 10mg/vial

Generic name : Kisspeptin-10

Sequence : Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂

Molecular weight : Approximately 1384.5 Da

Purity : ≥98.5% (HPLC)

Appearance : White lyophilized powder

Storage : Freeze at -20℃, protect from light, and store in a dry place.

Usage and Storage Guide

Kisspeptin-10 neuropeptide:

Kisspeptin-10 is a fully bioactive core decapeptide fragment of the kisspeptin family. As a key upstream regulator of hypothalamic gonadotropin-releasing hormone neurons, it plays a central role in the initiation and regulation of the reproductive axis. Unlike traditional hormones that act directly on the pituitary gland, it precisely regulates the pulsatile release of GnRH by specifically activating the GPR54 receptor, thereby controlling gonadotropin secretion. Its unique hypothalamic-level regulatory mechanism makes it irreplaceable in the study of puberty initiation, reproductive cycle regulation, sex hormone feedback, and reproductive-related disease models.

Due to its powerful GnRH-releasing stimulation and central role in the reproductive axis, Kisspeptin-10 is a preferred choice for many researchers in reproductive endocrinology and neuroendocrinology. It does not act directly on the gonads, but rather exerts its effects through precise neuroendocrine pathways, avoiding direct stimulation of peripheral organs. Its characteristic as a "master switch" for the reproductive system makes it an extremely important and specific probe tool in the study of hypogonadism, central precocious puberty, polycystic ovary syndrome, and reproductive dysfunctions related to energy balance.

Kisspeptin-10 neuropeptide applications:

Kisspeptin-10 specifically stimulates the pulsatile release of GnRH from the hypothalamus, thereby regulating the secretion of pituitary gonadotropins and initiating and maintaining reproductive function. Kisspeptin-10 is a key signal peptide based on neuroendocrine regulatory mechanisms and is a core molecule connecting metabolic state and reproductive function.

Kisspeptin-10 is widely used in research on reproductive physiology, neuroendocrinology, and the interaction between metabolism and reproduction. Its specific activation of the highest centers of the reproductive axis provides a crucial molecular tool for exploring the etiology of reproductive dysfunction, developing new reproductive regulation strategies, and understanding the mechanisms by which nutrition and stress affect reproduction. It also has important applications in research models of infertility, abnormal pubertal development, and related endocrine disorders.

Usage and storage methods

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvent : sterile water for injection or sterile physiological saline (0.9% NaCl)

Stability optimization : Sterile water containing 0.1% bovine serum albumin (BSA)

For intravenous/intravenous injection : sterile artificial cerebrospinal fluid or normal saline.

Standard preparation procedure :

Solvent preparation :

Solvents were sterile filtered through a 0.22 μm filter membrane.

The solvent temperature was restored to room temperature (20-25℃).

Avoid using diluents containing preservatives.

Reconstitution procedure :

Add 1.0 mL of the recommended solvent to a 10 mg vial.

Slowly pour the powder along the bottle wall to avoid direct impact on the powder.

Gently rotate the vial for 3-5 minutes until completely dissolved.

Final concentration: 10 mg/mL (approximately 7.2 mM)

Separate and store :

Immediately dispense into single-use doses of 10-20 μL using low-adsorption cryovials.

Clear labeling: Name, concentration, preparation date, batch number

Strictly avoid light

Working concentration/dosage reference :

In vitro cell experiments : Commonly used concentration range is 1 nM to 1 μM

Animal in vivo experiments (peripheral) : The usual dose range is 1 to 10 nmol/kg (intravenous/subcutaneous).

Intraventricular injection in animals : The usual dose range is 1 to 100 pmol/animal

Example of preparation calculation : If a 1 μM working solution is required, take 0.14 μL of stock solution and add it to 1 mL of culture medium.

Key points to note :

Dissolve gently : Avoid swirling or violent shaking; dissolve by gently rotating.

Strict aseptic technique : Operations are performed in a clean bench and sterile consumables are used.

Completely avoid light : It is sensitive to light, and all steps must be carried out under light-protected conditions.

Prepare and use immediately : Stability is limited after reconstitution, so it is recommended to use as soon as possible.

To avoid adsorption : use a solution containing 0.1% BSA or dilute with silanized low-adsorption tubes.

2. Storage conditions :

Unreconstituted lyophilized powder :

Long-term storage : Store frozen at -20℃, shelf life 24 months.

Optimal storage : Store frozen at -80℃, shelf life 36 months.

Strictly protected from light : Must be stored in the original light-proof aluminum foil bag.

Moisture protection : Keep dry; contains desiccant.

Reconstituted solution :

Use immediately : For best activity, use immediately after reconstitution.

Short-term storage : Store at 2-8℃ away from light for no more than 8 hours.

Repackage and freeze : Store at -20℃ or -80℃ away from light for no more than 1 week.

Repeated freeze-thaw cycles are strictly prohibited : a maximum of one freeze-thaw cycle will result in a significant reduction in potency.

Transportation conditions :

Freeze-dried powder : transported by dry ice (-78℃).

Short-term shipping : Transported with ice packs at 2-8℃, ensuring delivery within 48 hours.

Packaging : Light-proof aluminum foil bag + vacuum seal + foam insulated box.

3. Stability and Processing Standards :

Chemical and physical stability :

It is relatively stable in aqueous solutions with a pH of 5.0–7.0.

Avoid contact with strong acids, strong alkalis, oxidizing agents, and reducing agents.

It is sensitive to mechanical stress (such as violent blowing or vortex).

Maintenance of bioactivity :

Proper aliquoting and storage at -80°C are key to maintaining long-term activity.

Thaw the dispensed solution slowly on ice before use.

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