DSIP 2mg/vial Effectively Regulates Sleep Rhythm And Significantly Improves Sleep Quality

Delta Sleep-Inducing Peptide (DSIP) 2mg/vial: Key Information

Neuromodulatory nonapeptide | Endogenous sleep-regulating peptide 2mg/bottle

Generic name : Delta sleep-inducing peptide

English name : Delta Sleep-Inducing Peptide (DSIP)

Sequence : Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu

Molecular weight : Approximately 848.9 Da

Purity : ≥98.0% (HPLC)

Content : 2mg/bottle

Appearance : White lyophilized powder

Storage : Freeze at -20℃, protect from light, and store in a dry place.

Usage and Storage Guide

Delta sleep-inducing peptide (DSIP):

DSIP is an endogenous nonapeptide initially isolated from the cerebrospinal fluid of sleeping rabbits. Studies have shown it may be involved in regulating the sleep-wake cycle, particularly showing potential effects in promoting slow-wave sleep (Delta-wave sleep). As an exploratory neuropeptide research tool, it is thought to potentially regulate physiological sleep, stress response, and pain perception in experimental models by influencing the neuroendocrine system and neurotransmitter activity. Unlike traditional sedative-hypnotic drugs, its endogenous peptide properties and potential multi-system regulatory effects make it a standardized reagent of specific value in studying the fundamental mechanisms of sleep physiology, neuroendocrine function, and stress adaptation.

Due to its potential non-rapid eye movement (NREM) sleep regulation properties, DSIP is frequently chosen by researchers in sleep research, neuroendocrinology, and stress physiology. Compared to other exogenous sleep regulators, as an analogue of endogenous substances, it serves as a standardized research tool in exploring the mechanisms of physiological sleep initiation and maintenance, evaluating sleep deprivation models, and studying the effects of peptides on circadian rhythms and stress responses in animal experiments. In models studying the interactions between sleep and immunity, metabolism, and pain, its application provides a feasible experimental approach for exploring cross-system regulatory mechanisms.

Applications of Delta Sleep-Inducing Peptide (DSIP):

As an endogenous research peptide, it is used in specific experimental models to evaluate its potential effects on electroencephalographic patterns (especially Delta wave activity), sleep-wake structures, and related neuroendocrine markers. Its main applications are in exploring physiological sleep regulation mechanisms, the physiological effects of sleep deprivation, and the role of neuropeptides in stress adaptation.

It has been applied in sleep physiology and EEG research, chronic sleep deprivation models, stress-related behavior and endocrine studies, and exploratory experiments on pain perception modulation. Its well-defined structure and standardized experimental applications provide a fundamental research tool for studying the neurobiological basis of sleep and assessing the effects of sleep interventions on overall physiological function under controlled conditions.

Usage and Storage Methods

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvent : sterile water for injection or sterile sodium acetate buffer solution with pH 5.0-6.5.

Stability optimization : Sterile saline containing 0.1% bovine serum albumin (BSA)

For internal injection : dilute with sterile saline.

Important note : Avoid using alkaline solvents (pH > 8.0) or solutions containing organic solvents.

Standard preparation procedure :

Solvent preparation :

Use solvents that have been sterilely filtered through a 0.22 μm filter membrane.

The solvent temperature was restored to room temperature (20-25℃).

Reconstitution procedure :

Add 1.0 mL of the recommended solvent to a 2 mg vial.

Pour slowly along the bottle wall to avoid direct impact on the powder.

Gently rotate the vial for 3-5 minutes until completely dissolved.

Final concentration: 2 mg/mL (approximately 2.36 mM).

Dispensing and dilution :

Immediately dispense into single-use doses of 10-50 μL using low-adsorption cryovials.

Dilute to the required working concentration with sterile saline or a carrier solution containing 0.1% BSA.

Clearly label: name, concentration, preparation date, batch number.

Strictly avoid light during operation.

Working concentration/dosage reference :

In vitro cell/brain slice experiments : The commonly used concentration range is 0.1 nM to 1 μM, and preliminary experiments need to be performed to optimize the concentration based on the specific experimental system.

Animal in vivo experiments :

Intraperitoneal/intravenous injection : The usual dose range is 25 to 100 nmol/kg.

Intraventricular injection : The usual dose range is 1 to 10 nmol/animal.

It is strongly recommended that the precise dosage be determined based on the specific animal model, route of administration, research objectives, and published literature.

Example of preparation calculation : If a 100 nM working solution is required, take about 0.042 μL of stock solution and add it to 1 mL of solvent (gradual dilution is recommended).

Key points to note :

Dissolve gently : Avoid swirling or violent shaking; simply rotate gently to dissolve.

Strict aseptic technique : Operate in a clean bench and use sterile consumables, especially when administering drugs in vivo or centrally.

Completely avoid light : It is sensitive to light, and all steps must be carried out under light-protected conditions.

Prepare and use immediately : The stability is limited after reconstitution, so it is recommended to use it as soon as possible. After repackaging, store it under the specified conditions.

To avoid adsorption : dilution with a solution containing a carrier protein (such as 0.1% BSA) or silanized low-adsorption tubes can reduce adsorption on the tube walls.

2. Storage conditions :

Unreconstituted lyophilized powder :

Long-term storage : Freeze at -20℃, shelf life 24 months.

Optimal storage : -80°C deep freeze, shelf life 36 months.

Strictly protected from light : Must be stored in the original light-proof aluminum foil bag.

Moisture protection : Keep dry; contains desiccant.

Reconstituted solution :

Use immediately : For best activity, use immediately after reconstitution.

Short-term storage : Refrigerate at 2-8℃ away from light, for no more than 12 hours.

Repackage and freeze : Store at -20℃ or -80℃ away from light for no more than 72 hours.

Repeated freeze-thaw cycles are strictly prohibited : a maximum of one freeze-thaw cycle.

Transportation conditions :

Freeze-dried powder : transported by dry ice (-78℃).

Packaging : Light-proof aluminum foil bag + vacuum seal + foam insulated box.

3. Stability and Processing Standards :

Chemical and physical stability :

It is relatively stable in weakly acidic to neutral aqueous solutions.

Avoid contact with strong acids, strong alkalis, oxidizing agents, and reducing agents.

It is sensitive to high temperatures and mechanical stress (such as violent blowing or vortex).

Maintenance of bioactivity :

Proper aliquoting and storage at -80°C are key to maintaining long-term activity.

Thaw the dispensed solution slowly on ice before use.

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