CJC1295 Without DAC 2mg/vial Increase Muscle Mass, Improve Strength And Athletic Performance

CJC-1295 (without DAC) 2mg/vial core introduction

Growth hormone-releasing hormone analogues | Growth hormone-releasing peptide 2mg/vial

Generic name : CJC-1295 (Drug-free affinity complex)

English name : CJC-1295 (without DAC)

Sequence : Modified GRF(1-29)

Molecular weight : Approximately 3367.8 Da

Purity : ≥98.0% (HPLC)

Content : 2mg/bottle

Appearance : White lyophilized powder

Storage : Freeze at -20℃, protect from light, and store in a dry place.

Usage and Storage Guide

CJC-1295 (without DAC) growth hormone-releasing hormone analog:

CJC-1295 (DAC-free) is a modified growth hormone-releasing hormone (GHRH) analog composed of 29 amino acids, with a structure highly similar to endogenous GHRH. Unlike the long-acting version containing DAC, the DAC-free version has a shorter half-life, stimulating the pituitary gland to release endogenous growth hormone more closely to its natural physiological rhythm. In experimental studies, CJC-1295 (DAC-free) has shown a more precise ability to mimic the natural pulsatile secretion pattern of human growth hormone, making it an ideal research tool for studying the physiological release rhythm of growth hormone and assessing its role in muscle synthesis and lipid metabolism.

In exercise physiology and muscle metabolism research, CJC-1295 (without DAC) has attracted attention due to its ability to induce a growth hormone release pattern that more closely resembles physiological states. Researchers have used it in animal models and cell experiments to study the secretion kinetics of endogenous growth hormone and its specific mechanisms in promoting protein synthesis, increasing lean body mass, promoting lipolysis, and improving post-exercise recovery, providing scientific evidence for understanding the role of growth hormone in exercise adaptation and body composition optimization.

CJC-1295 (without DAC) Applications:

As a short-acting growth hormone-releasing hormone analog, it is primarily used to study the precise regulation of pulsatile growth hormone secretion and to evaluate its regulatory effects on muscle protein synthesis rate, lipid oxidation efficiency, muscle satellite cell activation, and overall energy metabolism in experimental models. It is a key experimental tool for studying the physiological rhythms of growth hormone and its role in exercise adaptation, muscle repair, and body composition improvement.

It is applied in exercise physiology research, muscle metabolism experiments, anabolism mechanism analysis, anti-aging related research, and growth hormone secretion rhythm research. It is used to study the potential effects of short-acting GHRH analogs on promoting muscle growth, reducing body fat, enhancing athletic performance, and accelerating the recovery process under strictly controlled experimental conditions.

Usage and Storage Methods

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvent : sterile water for injection

Carrier solution : diluted with sterile physiological saline for in vivo studies.

Important note : It has the best stability in neutral buffer solution (pH 7.2-7.6). Avoid using strong acid or strong base solutions.

Standard preparation procedure :

Solvent preparation :

Use sterile water for injection that has been aseptically filtered through a 0.22 μm filter membrane.

The solvent temperature was restored to room temperature (20-25℃).

Reconstitution procedure :

Add 1.0-2.0 mL of sterile water for injection to a 2 mg vial.

Pour slowly along the bottle wall to avoid direct impact on the powder.

Gently rotate the vial for 3-5 minutes until completely dissolved and the solution becomes clear.

Common final concentrations: 2 mg/mL or 1 mg/mL.

Dispensing and dilution :

Immediately dispense into single-use doses of 20-50 μL using low-adsorption cryovials.

Dilute with sterile saline to the required working concentration.

Clearly label: name, concentration, preparation date, batch number, and storage conditions.

Working concentration/dosage reference :

In vitro cell experiments : The commonly used concentration range is 0.1 nM to 100 nM, and preliminary experiments need to be performed to optimize the concentration based on the specific cell type and experimental purpose.

Animal experiments :

Subcutaneous injection : The usual dosage range is 1 to 5 μg/kg, and the frequency of administration is determined according to the research objectives.

It is strongly recommended that the precise dosage and dosing regimen be determined based on the specific animal model, route of administration, research objectives, and published literature.

Example of preparation calculation : If a 100 μg/mL working solution is required, take 0.05 mL of stock solution (2 mg/mL) and add it to 0.95 mL of physiological saline.

Key points to note :

Dissolve gently : Avoid swirling or violent shaking; simply rotate gently to dissolve.

Strict aseptic technique : Operate in a clean bench and use sterile consumables, especially when administering drugs in vivo.

Completely avoid light : It is sensitive to light, and all steps must be carried out under light-protected conditions.

Prepare and use immediately : The stability is limited after reconstitution, so it is recommended to use it as soon as possible. After repackaging, store it under the specified conditions.

To avoid adsorption : dilution with a solution containing 0.1% bovine serum albumin or silanized low-adsorption tubes can reduce adsorption on the tube walls.

2. Storage conditions :

Unreconstituted lyophilized powder :

Long-term storage : Freeze at -20℃, shelf life 24 months.

Optimal storage : -80°C deep freeze, shelf life 36 months.

Strictly protected from light : Must be stored in the original light-proof aluminum foil bag.

Moisture protection : Keep dry; contains desiccant.

Reconstituted solution :

Use immediately : For best activity, use immediately after reconstitution.

Short-term storage : Refrigerate at 2-8℃ away from light, for no more than 24 hours.

Repackage and freeze : Store at -20℃ or -80℃ away from light for no more than 1 week.

Repeated freeze-thaw cycles are strictly prohibited : a maximum of one freeze-thaw cycle.

Transportation conditions :

Freeze-dried powder : transported by dry ice (-78℃).

Short-term shipping : 2-8℃ ice pack shipping, ensuring delivery within 48 hours.

Packaging : Light-proof aluminum foil bag + vacuum seal + foam insulated box.

3. Stability and Processing Standards :

Chemical and physical stability :

It is relatively stable in neutral pH aqueous solutions and buffer solutions.

Avoid contact with strong acids, strong alkalis, and oxidizing agents.

It is sensitive to high temperatures and mechanical stress.

Maintenance of bioactivity :

Proper aliquoting and storage at -80°C are key to maintaining long-term activity.

Thaw and dispense the liquid slowly on ice or under refrigeration before use.

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