Description

Technical Parameters

Follistatin 344 1mg/vial core introduction

Pharmaceutical-grade peptide | Follicle-suppressing hormone 344 (Made in China)

Chemical name : Follicle-stimulating hormone 344 fragment

Sequence : Human follicle-stimulating hormone amino acid 1-344

CAS No .: 126853-54-1

Specification : 1mg/bottle (lyophilized powder)

Dosage form : Lyophilized peptide preparation for injection

Molecular weight : Approximately 38.5 kDa

Purity : ≥95% (RP-HPLC detection)

Bioactivity : Verified by activin binding inhibition assay

Storage temperature : -20℃ for long-term storage, 2-8℃ for short-term storage

Storage after reconstitution : Refrigerate at 2-8℃ and use within 24 hours.

Transportation conditions : Transport with ice packs or dry ice

Dissolving suggestion : Sterile water for injection or PBS buffer

Core Function | Core Effects

Follistatin 344 is a functional active fragment of follicle-stimulating hormone . As a natural binding protein of activin and myostatin , it has important scientific research value in muscle growth regulation, reproductive endocrinology research, and exploration of muscle disease treatment .

✅Biological mechanism :

Ligand binding : High affinity binding to activin A, activin B, and myostatin

Signal antagonism : blocking the binding of TGF-β family ligands to receptors

Muscle growth promotion : Relieves the inhibition of muscle growth by myostatin.

Multiple regulation : Involved in the regulation of multiple systems including reproduction, metabolism, and immunity.

✅Quality advantages of Chinese manufacturing :

Mammalian expression system : HEK293 or CHO cell expression, ensuring proper folding.

Ion exchange purification : multi-step chromatography purification to remove host proteins and endotoxins.

Endotoxin control : <1.0 EU/μg, meeting the standards for injectable protein.

Glycosylation modification : Maintaining the natural glycosylation pattern and preserving biological activity.

✅Quality Analysis Standards :

HPLC purity : RP-HPLC purity ≥ 95% (C18 column, acetonitrile-water gradient)

SEC-HPLC : Monomer content ≥90%

Mass spectrometry confirmation : MALDI-TOF confirms molecular weight

Western Blot : Validation of specific antibody recognition

Peptide mapping analysis : LC-MS/MS sequence confirmation after trypsin digestion

Product Features and Advantages

1. Scientific research application value :

Muscle biology research : Research on the regulatory mechanisms of muscle growth

Reproductive endocrinology research : Follicle development and hormone regulation

Disease model studies : Muscular dystrophy, Polycystic ovary syndrome

Drug Development : Preclinical Research on Drugs for the Treatment of Muscle Diseases

2. Technical parameters :

Expression system : Expression in mammalian cells, maintaining native conformation.

Glycosylation state : possessing natural N- and O-glycosylation modifications

Buffer system : 20 mM phosphate buffer, pH 7.2-7.4

Lyophilization protectant : Contains 5% trehalose, 0.01% Tween-80

Isoelectric point : pH 5.8-6.2

3. Advantages of Chinese manufacturing :

Large-scale production : Mature mammalian cell culture technology

Cost-effectiveness : It has a price advantage compared to imported products.

Quality control : Strictly implement cGMP-like production standards.

Technical Support : A professional team provides application guidance and technical consultation.

Customization services : We can provide different labels and different mutant versions.

Usage and Storage Guide

1. Storage conditions :

Unopened freeze-dried powder : can be stored for 18 months at -20℃, and for 24 months at -80℃.

Reconstituted solution : can be stored at 2-8℃ for 24 hours; avoid freezing.

Repackaging Recommendation : For long-term storage, it is recommended to repackage and freeze at -80℃.

Light protection requirements : Avoid strong light exposure, especially ultraviolet light.

Avoid repeated freeze-thaw cycles : It is recommended to divide the product into smaller portions and avoid more than two freeze-thaw cycles.

2. Reconstitution method :

Temperature equilibration : After removing from the freezer, allow to equilibrate to room temperature for 15-20 minutes.

Buffer selection : Sterile PBS (pH 7.4) or physiological saline is recommended.

Reconstitution concentration : Recommended initial concentration 0.5-1.0 mg/ml

Dissolving procedure : Slowly add buffer solution and gently vortex or rotate to dissolve.

Concentration determination : Protein concentration was determined using the BCA method.

Sterilization through filtration : using a 0.22μm low-protein adsorption filter membrane.

3. Recommendations for use in cell experiments :

Working concentration : Recommended concentration for cell experiments is 10-1000 ng/ml

Processing time : 24-72 hours, adjusted according to the experimental objective.

Positive control : It is recommended to set up an activator A treatment group.

Testing indicators :

Muscle-related gene expression (MyoD, Myogenin)

SMAD2/3 phosphorylation levels

Cell proliferation and differentiation status

4. Recommendations for use in animal experiments :

Dosage for mice : 1-10 mg/kg body weight

Administration routes : subcutaneous injection, intraperitoneal injection, or intramuscular injection

Dosage frequency : once daily or every other day

Experimental period : usually 2-4 weeks

Observation indicators :

Changes in body weight and muscle mass

muscle fiber cross-sectional area

Motor function test

Blood biochemical indicators

5. In vitro binding assay :

ELISA method :

Coated with activator A, detecting Follistatin binding

Competitive ELISA detection of myostatin binding

SPR/BLI analysis :

Determining the binding kinetics of ligands

KD values are typically in the pM-nM range.

Pull-down experiment :

Verify the interaction with the target protein

Combined with specificity verification

Research and application directions

1. Research on muscle diseases :

Exploration of treatment for Duchenne muscular dystrophy

Pathogenesis of muscular dystrophy

cachexia-related muscle wasting

Age-related sarcopenia

2. Reproductive medicine research :

Mechanism of Polycystic Ovary Syndrome

Follicle development regulation

Reproductive endocrine disorders

New strategies for treating infertility

3. Metabolic research :

Muscle-fat metabolic interaction

Insulin sensitivity regulation

Energy metabolism balance

Obesity-related metabolic disorders

4. Developmental Biology :

Embryonic muscle development

Regulation of organogenesis

Tissue regeneration mechanism

Stem cell differentiation regulation

Why choose Chinese-made Follistatin 344?

High purity : HPLC purity ≥95%, ensuring experimental accuracy.

High activity : Expressed in mammalian cells, maintaining native conformation and function

Strict quality control : Multi-dimensional quality control ensures high batch-to-batch stability.

Complete documentation : Provides complete documentation including COA, MSDS, and analysis certificates.

Technical Support : A professional team provides support for experimental design and results analysis.

Summarize

This product is high-purity recombinant human Follistatin 344 , produced using advanced mammalian cell expression and protein purification technology in China . It provides a high-quality, highly active research tool for muscle biology, reproductive endocrinology, and developmental biology research . Proper storage conditions, standardized operating procedures, and reasonable experimental design are crucial to ensuring the reliability and reproducibility of research results.

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