Melatonin 10mg/vial Effectively Improve Sleep Quality

Melatonin 10mg/bottle core introduction

High-purity melatonin raw material | Made in China, pineal hormone 10mg/bottle

Generic name : Melatonin

Chemical name : N-acetyl-5-methoxytryptamine

English name : Melatonin

CAS No .: 73-31-4

Molecular formula : C₁₃H₁₆N₂O₂

Molecular weight : 232.28 g/mol

Melting point : 116-118℃

Appearance : White to off-white crystalline powder

Purity : ≥99.5% (HPLC)

Specific curl : Meets the requirements

Storage : Refrigerate at 2-8℃, away from light, sealed, and stored in a dry place.

Usage and Storage Guide

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvents : anhydrous ethanol or DMSO (dimethyl sulfoxide)

Secondary solvents : propylene glycol , PEG400

Water-soluble preparation : First dissolve in a small amount of ethanol or DMSO, then add physiological saline containing 0.1% Tween-80.

Standard preparation procedure :

Preparation of stock solution :

Accurately weigh 10mg of melatonin using an analytical balance.

Add 1.0 mL of anhydrous ethanol and gently vortex until completely dissolved.

A stock solution of 10 mg/mL (approximately 43 mM) was obtained.

Preparation of working solution :

In vitro cell experiments : Dilute with culture medium to a working concentration of 0.1-10 mM.

Animal experiments : Dilute with physiological saline containing 0.1% Tween-80

Calculation formula: Required concentration × Required volume ÷ Stock solution concentration

Separate and store :

Immediately dispense into 50-100μL portions.

Labeling concentration, preparation date, and batch number.

Key points to note :

Dissolving Tips : Melatonin has extremely low solubility in water (approximately 0.1 mg/mL), and must be dissolved using an organic solvent first.

Light protection : All operations must be performed under light-protected conditions.

Temperature control : Avoid high temperatures during the preparation process.

Aseptic technique : Aseptic technique is required when used in cell or animal experiments.

Stability : The stock solution remains stable for several months when stored at -20°C in the dark.

2. Storage conditions :

Unopened raw materials :

Short-term storage : Refrigerate at 2-8℃, away from light. Shelf life: 24 months.

Long-term storage : Freeze at -20℃, protect from light, shelf life 36 months.

Moisture-proof storage : Built-in desiccant, relative humidity ≤60%.

The prepared solution is as follows :

Stock solution : Store frozen at -20℃ away from light; shelf life 3-6 months.

Working solution : Prepare fresh before use, store at 2-8℃ protected from light for no more than 24 hours.

Conditions to avoid : Avoid repeated freeze-thaw cycles, avoid sunlight.

Transportation conditions :

Cold chain transportation : refrigerated transport at 2-8℃

Light-proof packaging : sealed aluminum foil bag

Shockproofing : transported in foam boxes with ice packs.

3. Preparation Notes :

Solvent selection principles :

The research objective determines the choice of solvent.

DMSO is the preferred choice for in vitro studies.

The propylene glycol/Tween-80 system is the preferred method for in vivo studies.

Dissolving steps :

Add a small amount of organic solvent first

Add the aqueous phase after complete dissolution.

Avoid dissolving directly in water

Concentration control :

Stock solution concentration 10 mg/mL

The working solution concentration is based on the experimental design.

The final concentration of the organic solvent is ≤0.1%.

Stability protection :

Avoid light operation

Low temperature storage

Avoid oxidation

Store in an airtight container

4. Research and application formulation :

Cellular experiments :

Stock solution: 10 mg/mL DMSO solution

Working concentration: 0.1-10mM

Final DMSO concentration: ≤0.1%

Animal experiments :

Intraperitoneal injection: Prepared with physiological saline containing 0.5% sodium carboxymethyl cellulose.

Oral administration: Prepared with corn oil or olive oil.

Dosage range: 1-50 mg/kg

In vitro biochemical experiments :

Prepare directly with anhydrous ethanol or DMSO

The concentration should be adjusted according to experimental requirements.

Note the solvent comparison.

Quality Advantages of Chinese Manufacturers

This product is manufactured by a leading Chinese API (Active Pharmaceutical Ingredient) manufacturer, complies with cGMP standards, and is designed specifically for research and the pharmaceutical industry.

✅Advanced production technology :

Chemical synthesis route : using 5-methoxytryptamine acetylation process

Chirality control : Ensure optical purity ≥ 99.9%

Crystallization and refining : Multiple recrystallizations to improve purity

Fully enclosed production : prevents contamination and degradation

✅Strict quality control :

Purity testing :

HPLC purity ≥ 99.5%

Single impurity ≤0.1%

Total impurities ≤ 0.5%

Structural confirmation :

Infrared spectroscopy (IR) analysis

Nuclear magnetic resonance (NMR) proton/carbon spectrum

Mass spectrometry (MS) molecular weight confirmation

Physicochemical properties :

Melting point: 116-118℃

Specific curl: -25° to -30°

Loss on drying: ≤0.5%

Impurity control :

Related substance: 5-methoxytryptamine ≤0.1%

Heavy metals: ≤10ppm

Residual solvent: Complies with ICH requirements

Residue on ignition: ≤0.1%

Microbial testing :

Total bacterial count: ≤100 CFU/g

Mold and yeast: ≤10 CFU/g

Escherichia coli: Not detectable

Salmonella: Not detectable

✅Stability study :

Influencing Factors Experiment :

High temperature: 60℃, stable for 10 days

High humidity: Stable for 10 days at RH 92.5%

Light intensity: 4500 Lx, stabilized after 10 days

Accelerated testing : Stable for 6 months at 40℃/RH75%.

Long-term test : Stable for 24 months at 25℃/RH60%.

Solution stability :

Ethanol solution is stable for 6 months when stored at -20°C.

DMSO solution is stable for 6 months when stored at -20℃.

✅Professional Packaging :

Packaging materials : amber glass bottle + butyl rubber stopper + aluminum-plastic cap

Nitrogen purging protection : to prevent oxidative degradation

Drying process : Built-in molecular sieve desiccant

Light-proof design : sealed with an outer aluminum foil bag

Complete information : The label includes batch number, purity, and expiration date.

✅Technical Support :

Provide complete quality documentation

Including COA, MSDS

Technical consultation and support

Customized specifications service

Need anything, please contact us

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