HGH 191aa 10iu/vial Comprehensive Promotion Of Growth, Repair And Metabolic Optimization

Growth Hormone (HGH) 191aa 10 IU/bottle (Core Introduction)

Human growth hormone | 191 amino acid sequence recombinant protein 10 IU/bottle

Generic name : Recombinant human growth hormone

Sequence : A single-chain polypeptide of 191 amino acids

Molecular weight : Approximately 22.1 kDa

Purity : ≥98.0% (SEC-HPLC)

Specific activity : ≥3.0 IU/mg

Appearance : White lyophilized powder

Storage : Refrigerate at 2-8℃, away from light and stored in a dry place.

Usage and Storage Guide

Growth hormone (HGH) 191aa:

Human growth hormone (HGH) is a single-chain polypeptide hormone composed of 191 amino acids, produced using recombinant DNA technology. It has the exact same amino acid sequence and biological activity as the growth hormone secreted by the human pituitary gland. Unlike anabolic steroids, HGH directly activates growth hormone receptors on the cell surface, thereby stimulating the liver to produce insulin-like growth factor 1 (IGF-1), thus exerting a wide range of physiological effects, including promoting protein synthesis, lipolysis, bone growth, and cell regeneration. Its comprehensive pro-anabolism and metabolic regulation functions make it a core research asset in the study of growth and development, metabolic regulation, tissue repair, and anti-aging mechanisms.

Due to its well-established growth-promoting and metabolic-regulating effects, recombinant human growth hormone (rHH) is a preferred choice for researchers in endocrinology, exercise physiology, and regenerative medicine. It does not act through androgen or estrogen receptors, but rather through the classic GH-IGF-1 axis, avoiding the risks of sex hormone-related side effects. Its ability to improve body composition, promote wound healing, and regulate overall metabolism makes it a standardized and reliable research tool in animal models of growth disorders, metabolic syndrome, sarcopenia, and wound repair.

Growth hormone (HGH) 191aa Applications:

It activates growth hormone receptors, stimulates the liver to synthesize and release insulin-like growth factor 1, and comprehensively promotes protein synthesis, fat breakdown, bone growth, and organ development. Recombinant human growth hormone is a key protein hormone with broad-spectrum anabolic and catabolic regulatory functions.

Recombinant human growth hormone is widely used in preclinical studies of growth and development, metabolism, body composition regulation, and tissue regeneration and repair. Its well-defined mechanism of action and diverse physiological effects provide an important experimental model for studying growth hormone deficiency, exploring age-related metabolic changes, evaluating anabolic therapies, and promoting recovery after surgery or trauma. It also plays a crucial role in basic research in sports science, nutrition, and geriatrics.

Usage and storage methods

1. Reconstitution and preparation :

Recommended solvent :

Specified solvent : The accompanying sterile water for injection or the matching dedicated solvent.

Important Note : Do not use ordinary physiological saline, water for injection containing preservatives, or any other buffer solution . Incorrect solvents may alter osmotic pressure or pH, leading to protein denaturation, precipitation, or loss of activity.

For experimental dilution : After dissolving in the above-mentioned special solvent, it can be appropriately diluted with sterile phosphate buffer at pH 7.4 according to experimental requirements.

Standard preparation procedure :

Solvent preparation :

Remove the accompanying sterile water for injection from the refrigerator and let it stand to room temperature.

The entire procedure was performed in strict accordance with aseptic principles and within a biosafety cabinet.

Reconstitution procedure :

Add 0.5-1.0 mL of the specified sterile water for injection to a 10 IU vial.

With the needle angled downwards, slowly inject the solvent along the bottle wall, avoiding direct impact on the lyophilized powder.

Gently rotate the vial horizontally in the palm of your hand . Do not shake, vigorously agitate, or vortex it until the powder is completely dissolved and the solution is clear.

Preparation concentration: Add 1.0 mL of solvent to obtain a solution of 10 IU/mL.

Repackaging and Use :

The reconstituted solution should be used as soon as possible. If short-term storage is necessary, it should be placed in a refrigerator at 2-8°C and used within 24 hours.

Calculate the dispensing volume according to the experimental requirements and use sterile, low-adsorption centrifuge tubes.

Working concentration/dosage reference :

Animal in vivo experiments : The commonly used dosage range is 0.1 - 1.0 IU/kg/day (subcutaneous injection). The specific dosage needs to be determined based on the animal model and experimental purpose.

In vitro cell experiments : The commonly used concentration range is 10-100 ng/mL, which needs to be optimized according to the cell type.

Important note : Dosage must be accurately titrated through preliminary experiments. Overuse may lead to metabolic disorders such as insulin resistance.

Key points to note :

Solvent specificity : The supplied sterile water for injection must be used .

Dissolving method : Dissolve only by gently rotating horizontally ; vigorous handling will cause protein denaturation and inactivation.

Aseptic technique : Aseptic technique must be performed in a clean bench or biosafety cabinet.

Observe the solution : The reconstituted solution should be clear and colorless. If turbidity, flocculent matter, or discoloration occurs, the solution must be discarded.

Ready-to-use : It should be used as soon as possible after reconstitution. Even if refrigerated, its activity will decrease over time.

2. Storage conditions :

Unreconstituted lyophilized powder :

Long-term storage : Refrigerate at 2-8℃ . Shelf life is 24 months (subject to product label). Freezing and lyophilizing powder is strictly prohibited .

Strictly protected from light : Must be stored in the original light-proof outer box.

Moisture protection : Keep dry; contains desiccant.

Reconstituted solution :

Use immediately : It should be used immediately after reconstitution for optimal activity.

Short-term storage : If not used immediately, it can be refrigerated at 2-8℃, but must be used within 24 hours .

Freezing is strictly prohibited : Reconstituted solutions must not be frozen , as freezing will destroy their spatial structure, leading to irreversible aggregation and inactivation.

Transportation conditions :

Cold chain transportation : Must be transported under refrigerated conditions of 2-8℃, using sufficient ice packs and insulated boxes.

Packaging : Light-proof outer box + high-strength insulated box + temperature monitor.

3. Stability and Processing Standards :

stability :

It is highly sensitive to temperature, pH, mechanical shear force, and surface adsorption.

It is relatively stable under the right solvent and pH conditions, but its stability decreases significantly after dissolution.

Key points for preserving active ingredients :

Strict adherence to refrigerated (non-frozen) storage conditions is key to maintaining long-term viability.

Gentle handling and avoiding repeated freeze-thaw cycles are essential for maintaining reconstituted activity.

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