Description

Technical Parameters

Botox Type A 100 IU/vial: Key Features

Botulinum toxin type A neurotoxin complex | Neuromuscular junction blocking protein 100 IU/bottle

Active ingredient : Botulinum toxin type A

Source : Purified by fermentation of Clostridium botulinum

Molecular weight : Approximately 150 kDa

Purity : ≥99.5% (SDS-PAGE)

Specific activity : ≥100 IU/vial

Appearance : White freeze-dried cake or powder

Storage : Freeze at -20℃, protect from light, and store in a dry place.

Usage and Storage Guide

Botox type A neurotoxin:

Botulinum toxin type A is a highly potent neurotoxin that irreversibly blocks the release of acetylcholine by specifically cleaving the SNAP-25 protein at nerve endings, leading to chemical denervation and temporary paralysis of the target muscle. Unlike ordinary peptides or hormones, its effects are precise, long-lasting, and highly specific. Its unique neuromuscular junction blocking mechanism makes it irreplaceable in fundamental neurobiological fields such as muscle spasms, autonomic hyperactivity, pain signal transduction, and neurotransmitter release. It is also a core tool for studying the functional regulation of facial expression muscles, skeletal muscles, and smooth muscles.

Due to its recognized potent neuromuscular blocking effect and precise local action, botulinum toxin type A is the preferred choice for many researchers in neuroscience, exercise physiology, and pain research. It does not act through systemic metabolism or hormonal pathways, but rather precisely targets cholinergic nerve endings, avoiding widespread systemic side effects. Its controllable, dose-dependent local muscle relaxation and glandular secretion inhibition make it a standardized and quantifiable key research tool in animal models of hyperactivity syndrome, neuropathic pain, hyperhidrosis, and related diseases.

Applications of Botox type A:

It specifically cleaves the SNAP-25 protein at nerve endings, irreversibly blocking the release of acetylcholine, leading to chemical denervation and temporary relaxation of the target muscle. Botulinum toxin type A is a highly effective neuromodulator based on a precise molecular mechanism and is the gold standard tool for studying neuromuscular and autonomic nervous system function.

Botulinum toxin type A is widely used in basic neuroscience, exercise physiology, pain medicine, and research on the pathological mechanisms of related diseases. Its well-defined target and controllable pharmacological effects provide a unique research window for exploring the physiology of the neuromuscular junction, the pathology of muscle spasms, abnormal glandular secretion, and the mechanisms of chronic pain. It is also crucial in evaluating novel antispasmodic drugs and studying muscle plasticity and nerve regeneration.

Usage and storage methods

1. Reconstitution and preparation :

Recommended solvent :

The only specified solvent is the accompanying sterile, preservative-free physiological saline (0.9% NaCl).

Absolutely prohibited : The use of preservative-containing saline, sterile water for injection, lactated Ringer's solution or any other unspecified solution is strictly prohibited , as it may lead to toxin inactivation, aggregation or unpredictable biological effects.

Standard preparation procedure :

Solvent preparation :

Remove the accompanying sterile saline solution from the refrigerator (2-8℃) and let it stand until it reaches room temperature.

The entire procedure was performed in strict accordance with aseptic principles and within a biosafety cabinet.

Reconstitution procedure :

Add the specified volume (e.g., 1.0 mL, 2.0 mL, or 4.0 mL) of the designated sterile saline solution to a 100 IU vial.

With the needle angled downwards, slowly inject the solvent along the bottle wall to avoid directly impacting the freeze-dried cake and creating air bubbles.

Gently rotate the vial horizontally in the palm of your hand , without shaking or agitating, until the lyophilized substance is completely dissolved and the solution is clear and colorless.

Concentration to be prepared: Determined according to experimental requirements (e.g., adding 2.0 mL of solvent to obtain a 50 IU/mL solution).

Repackaging and Use :

The reconstituted solution should be used immediately . If short-term storage is necessary, it should be placed in a refrigerator at 2-8°C and used within 4 hours.

Do not repackage and freeze reconstituted solutions.

Working concentration/dosage reference :

Animal experiments (local intramuscular injection) : The dosage needs to be precisely calculated based on the animal species, weight, target muscle volume and experimental purpose, and is usually in the range of 0.5-5 IU/kg . It must be determined through preliminary experiments.

In vitro cell experiments : When used to study the mechanism of neurotransmitter release, extremely low concentrations (usually in the pM to nM range) must be used under professional guidance.

Important Note : Botulinum toxin is extremely potent, with a steep dose-response curve. Dosage calculations and administration must be performed by professionally trained personnel .

Key points to note :

Solvent exclusivity : Only the supplied sterile, preservative-free physiological saline solution must be used .

Dissolving method : Dissolve only by gently rotating horizontally . Any vigorous operation may cause protein denaturation and loss of activity.

Aseptic technique : Must be performed in a biosafety cabinet, using sterile syringes, needles and consumables.

Observe the solution : After reconstitution, it should be a clear, colorless solution without visible particles. If it becomes cloudy, discolored, or contains particles, it must be discarded.

Ready-to-use : Stability drops sharply after reconstitution, and must be used immediately or within a specified very short time.

2. Storage conditions :

Unreconstituted lyophilized powder :

Long-term storage : Store frozen at -20℃, shelf life 24 months (subject to product label).

Optimal storage : Store at -80°C for further extended stability.

Strictly protected from light : Must be stored in the original light-proof outer box.

Keep dry.

Reconstituted solution :

Use immediately : It should be used immediately after reconstitution for optimal activity.

Short-term storage : If not used immediately, it can be refrigerated at 2-8℃, but must be used within 4 hours .

Freezing is strictly prohibited : Reconstituted solutions must not be frozen , as freezing will completely destroy their structure and lead to permanent inactivation.

Transportation conditions :

Cold chain transportation : Must be transported under conditions of dry ice (-78℃) or below -20℃.

Packaging : Light-proof outer box + vacuum seal + high-strength insulated box + temperature monitor.

3. Stability, safety, and handling procedures :

stability :

It is highly sensitive to temperature, pH and mechanical shear force.

It is relatively stable in the pH environment of dedicated sterile saline, but it will still degrade rapidly after dissolution.

Biosafety :

Biohazard level : Botulinum toxin is a high-risk biological agent , and its handling must comply with national regulations on biosafety management of laboratories handling highly pathogenic microorganisms.

Personal protective equipment : Operators must wear protective clothing, gloves, goggles and masks, and the operation must be carried out in a biosafety level 2 cabinet (BSC-II).

Waste disposal : All consumables and remaining solutions that have come into contact with toxins must be soaked in a high-concentration chlorine-containing disinfectant (such as 1% sodium hypochlorite) for at least 30 minutes, and then subjected to high-pressure steam sterilization.

Emergency Response : Develop and be familiar with emergency plans for leaks and personnel exposure. Laboratories should keep antitoxin serum readily available.

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