GDF-8 1mg/vial Significantly Promotes Muscle Mass And Strength Improvement

GDF-8 1mg/bottle core introduction

Myostatin | Transforming Growth Factor-β Superfamily Member 1mg/vial

Generic name : Myostatin (Growth Differentiation Factor-8)

English name : Myostatin (Growth Differentiation Factor 8, GDF-8)

Protein structure : Mature peptides are homodimers, with each chain containing approximately 110 amino acids.

Molecular weight : Approximately 25 kDa (monomer form)

Purity : ≥90% (SDS-PAGE/HPLC)

Content : 1mg/bottle

Appearance : White lyophilized powder

Storage : Freeze at -80℃, protect from light, and store in a dry place.

Usage and Storage Guide

GDF-8 (myostatin):

GDF-8, a member of the transforming growth factor-β superfamily, is primarily expressed in skeletal muscle. As a negative regulator of muscle growth, it precisely controls skeletal muscle mass by binding to the activin receptor IIB/ALK4/5 signaling pathway, inhibiting myoblast proliferation and differentiation. As a key negative feedback signaling protein for muscle growth, it plays a central role in experimental models of muscle development, regeneration, atrophy (such as cancer cachexia and age-related sarcopenia), and metabolic diseases. Compared to pro-anabolism factors, its negative regulatory characteristics provide a unique perspective for understanding the balance mechanisms of muscle homeostasis.

Due to its crucial negative regulatory role in muscle growth, GDF-8 is widely used by researchers in muscle biology, regenerative medicine, exercise physiology, and metabolic diseases. Compared to gene knockout models, its recombinant protein form provides a standardized experimental tool for precisely controlling the intensity of inhibitory signals, studying dose-response and time processes in vitro and in vivo. Its application in studies of muscle regeneration disorders, models of muscle atrophy, and metabolic research related to muscle-fat interactions provides a foundation for evaluating potential intervention strategies targeting the GDF-8 pathway.

GDF-8 Applications:

As a classic negative regulator of muscle growth, it is used in in vitro and in vivo models to accurately simulate or enhance muscle growth inhibitory signals, thereby studying its inhibitory effects on myoblast proliferation, differentiation, skeletal muscle mass, and function. It is primarily used to explore the molecular mechanisms of muscle atrophy, impaired muscle regeneration, and the interactions between muscle and other tissues (such as fat and bone).

It is widely used in models of muscular atrophy, studies of myoblast/satellite cell function, muscle regeneration experiments, and research on muscle metabolic disorders related to obesity and diabetes. Its standardized recombinant protein form provides a core research tool for evaluating novel anti-muscular atrophy drugs, studying the molecular basis of exercise adaptation, and exploring therapeutic strategies targeting GDF-8 signaling.

Usage and Storage Methods

1. Reconstitution and preparation :

Recommended solvent :

Preferred solvent : Dilute acetic acid solution with pH 4.0-5.0 (e.g., 20mM acetic acid).

Key carrier : The carrier protein must be added to the solvent and all diluents ; a sterile acetic acid solution containing 0.1% bovine serum albumin (BSA) is recommended.

Working solution dilution : Dilute to physiological pH with sterile PBS or cell culture medium containing 0.1-1% BSA.

Absolutely prohibited : It is strictly forbidden to directly dissolve or dilute the protein using pure water, neutral PBS, or solutions without carrier proteins, otherwise irreversible protein aggregation, precipitation, and inactivation will occur.

Standard preparation procedure :

Solvent preparation :

Prepare a 20mM sterile acetic acid solution containing 0.1% BSA (or higher purity carrier protein) and filter it through a 0.22μm filter membrane.

The solvent needs to be pre-cooled on ice before use.

Reconstitution procedure :

Perform the procedure on ice. Add 1.0 mL of pre-chilled acidic carrier protein solvent to a 1 mg vial.

Use a micropipette to inject the sample very slowly along the bottle wall, avoiding the generation of air bubbles or direct impact on the freeze-dried cake.

Place the vial on ice and gently rotate it horizontally for 5-10 minutes until the powder is completely dissolved. Do not vortex, shake, blow on, or violently agitate .

Final concentration: 1 mg/mL (approximately 40 μM) acidic stock solution.

Dispensing and gradient dilution :

Immediately dispense into single doses of 2-10 μL using low-adsorption silanized centrifuge tubes on ice.

When diluting, first dilute the acidic stock solution at least 100 times with PBS or culture medium containing 0.1-1% BSA to neutralize the pH. For example, take 1 μL of stock solution and add it to 99 μL of PBS containing 1% BSA to obtain an intermediate solution of 10 μg/mL.

The intermediate solution is then diluted to the final working concentration. All dilution steps are performed on ice.

Clearly labeled: GDF-8, concentration, pH, preparation date, batch number, storage temperature.

Working concentration reference :

In vitro cell experiments : The commonly used concentration range is 0.1 to 5 μg/mL (approximately 4 to 200 nM), which needs to be optimized according to cell type and experimental purpose.

In vivo animal experiments : The dosage must be strictly determined according to the model and the route of administration (e.g., intraperitoneal, intramuscular, intravenous) based on the literature. The commonly used range is in the μg/kg to mg/kg range, and preliminary experiments must be conducted .

Calculation example : To prepare a 100 ng/mL working solution, take 1 μL of the 10 μg/mL intermediate solution and add it to 100 μL of culture medium.

Key points to note :

Low temperature and gentleness : The entire process must be carried out on ice, and the movements must be extremely gentle to prevent protein denaturation.

Forced acid dissolution : It must be dissolved in an acidic buffer solution (pH 4-5) , which is crucial for maintaining its solubility and activity.

Forced carrier proteins : Dissolution and dilution must be performed using solutions containing carrier proteins such as BSA to prevent adsorption and aggregation.

Freeze-thaw cycle strictly prohibited : Repeated freeze-thaw cycles are strictly prohibited ; the solution must be dispensed in one go. Diluted working solution must not be stored ; it must be prepared and used immediately.

Observation of the condition : After reconstitution, the solution should be clear and slightly opalescent. The presence of any flocculent matter, particles, or turbidity indicates that the protein has denatured and aggregated, and the solution must be discarded.

2. Storage conditions :

Unreconstituted lyophilized powder :

Storage only : -80°C (deep freeze ). Do not store at -20°C.

Shelf life : 12 months at -80℃.

Strictly protect from light : Store in the original light-proof aluminum foil bag with a desiccant inside.

Reconstituted solution :

Immediate dispensing : Dispensing must be completed within 5 minutes after reconstitution .

Repackaged and frozen : Can only be stored at -80℃ , maximum shelf life 2 weeks, not recommended for storage.

Storage is strictly prohibited : The diluted working solution must not be stored and must be kept on ice and used within 4 hours.

Transportation conditions :

It must be transported under dry ice (-78℃) conditions.

The packaging must have sufficient dry ice and insulation to ensure that it does not thaw throughout the process.

3. Stability and Processing Standards :

Physicochemical stability :

It is pH sensitive and is only stable and soluble at acidic pH (4.0-5.0) . It readily aggregates and precipitates at neutral and alkaline pH.

It is extremely sensitive to thermal and mechanical stress (vortex, oscillation, bubbling) and repeated freeze-thaw cycles.

It is sensitive to surface adsorption, so a carrier protein and a low-adsorption container must be used.

Maintaining bioactivity :

four principles: " acidic dissolution, carrier protection, low-temperature gentleness, and strict prohibition of freeze-thaw cycles ".

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