N-Acetyl Epitalon Amidate 5mg/vial Regulating Biological Rhythms And Slowing Down The Aging Process

N-Acetylepitalonamide 5mg/bottle - Core Introduction

Research-grade telomerase-activating peptide | Made in China anti-aging tetrapeptide 5mg/bottle

Generic name : N-acetylepitalonamide

Amino acid sequence : Ac-Ala-Glu-Asp-Gly-NH₂

CAS No .: 307297-39-8

Molecular formula : C₁₅H₂₃N₅O₈

Molecular weight : 401.38 g/mol

Purity : ≥99.0% (HPLC detection)

Appearance : White to off-white lyophilized powder

Storage : Store frozen at -20℃, protected from light; after thawing, store at -20℃.

Core advantages and functions

N-acetylepitalonamide is a chemically modified epitalon analogue with an N-terminal acetyl group and C-terminal amidation, bearing the sequence Ala-Glu-Asp-Gly . This modification significantly enhances the peptide's metabolic stability, cell membrane permeability, and bioavailability . As a telomerase activator and epigenetic regulator , this product demonstrates breakthrough potential in anti-aging research, telomere biology, cellular senescence, tissue regeneration, and lifespan extension by activating telomerase, upregulating the expression of telomere-related genes, and regulating DNA methylation patterns . It is a cutting-edge tool in modern aging biology and regenerative medicine research .

✅Core Mechanism of Action :

Telomerase activation : specifically activates telomerase reverse transcriptase (TERT) expression.

Telomere elongation : promotes telomere DNA synthesis and delays telomere shortening.

Epigenetic regulation : regulating DNA methylation patterns in aging-related genes

Antioxidant defense : Enhances the activity of antioxidant enzymes and reduces the accumulation of reactive oxygen species.

Enhanced autophagy : Promotes cellular autophagy and clears damaged organelles.

Stem cell function : Improves the proliferation and differentiation potential of senescent stem cells

Gene stability : reducing the accumulation of DNA damage and maintaining genome stability

✅Quality advantages of Chinese manufacturers :

Solid-phase synthesis process :

Microwave-assisted solid-state synthesis of Fmoc

Acetylation and amidation modification efficiency ≥95%

Synthesis yield ≥80%, batch production up to gram level

Purification and quality control :

Three-dimensional purification: ion exchange → molecular sieve → reversed-phase HPLC

Purity control: Main peak purity ≥ 99.0%, single impurities ≤ 0.3%

Chiral control: D-type amino acid content ≤ 0.1%

Modification Validation: Mass Spectrometry Confirms Acetyl and Amide Modifications

Quality Analysis :

Peptide content: ≥98.0% (amino acid analysis)

Moisture content: ≤3.0% (Karl Fischer method)

Acetate content: ≤8.0% (ion chromatography)

Residual solvent: Complies with ICH Q3C standards

Endotoxin: <5 EU/mg

Activity verification :

Telomerase activity: TRAP assay to verify telomerase activation

Cellular senescence: SA-β-gal staining verifies anti-aging effects

Gene expression: qPCR detection of TERT, p16, and p21 expression

Stability: Plasma stability ≥ 4 hours

✅Product Features :

Excellent stability : acetylation/amidation modification significantly enhances stability.

Cell permeability : 3-5 times higher than natural peptides in cellular uptake.

Bioavailability : Subcutaneous injection bioavailability ≥85%

Long-lasting effect : Plasma half-life extended to 2-3 hours

Good safety profile : no genotoxicity, no teratogenicity.

Usage and Storage Guide

1. Reconstitution and preparation :

Recommended solvents :

Preferred solvent : sterile physiological saline

Cell experiments : sterile DMSO (final concentration <0.1%) or complete culture medium

In vivo studies : sterile saline or saline containing 0.1% BSA.

Standard configuration :

Add 1 ml of sterile saline solution and gently vortex to dissolve.

A stock solution of 5 mg/ml (approximately 12.5 mM) was obtained.

Dilute to working concentration with appropriate solvent.

Avoid vigorous shaking to prevent peptide chain breakage.

Recommended working concentration :

Cellular experiments: 0.1-10 μM

Animal experiments: 10-100 μg/kg

Long-term in vivo dosage: 0.1-1 mg/kg

In vitro enzyme activity: 1-100 nM

Precautions :

Use sterile, protease-free solution for dissolution.

The operation is carried out on ice to reduce degradation.

Prepare and use immediately to avoid repeated freeze-thaw cycles.

Avoid using strong acid or strong alkali solutions.

2. Storage conditions :

Lyophilized powder :

Store at -20℃ in a cool, dry place away from light.

36 months under these conditions

Avoid repeated freeze-thaw cycles

Solution state :

It can be stored at 4℃ for 24 hours

It can be stored at -20℃ for 1 month (when stored in smaller portions).

Avoid repeated freeze-thaw cycles (maximum 3 times).

Transportation conditions :

Dry ice transport (-78℃)

For short-term transport, ice packs can be used for preservation.

Avoid high temperatures, sunlight, and humidity.

3. Research and application plan :

In vitro studies :

Cell model :

Human mesenchymal stem cells

Human umbilical vein endothelial cells

Human fibroblasts

Neural stem cells

Processing conditions :

Pretreatment: Evaluation 24-48 hours after peptide treatment

Long-term treatment: Change the peptide-containing culture medium every 3-4 days.

Concentration gradient: 0.1, 1, 10 μM recommended.

Testing indicators :

Telomerase activity: TRAP assay

Telomere length: qPCR or Southern blot

Aging marker: SA-β-gal staining

Proliferation capacity: Colony formation experiment

Animal in vivo studies :

Aging Model :

Naturally aging mice

Premature aging mouse model

Radiation-induced aging model

D-galactose-induced aging model

Dosage regimen :

Subcutaneous injection: 0.1-1 mg/kg, once daily.

Intraperitoneal injection: 0.5-2 mg/kg, once daily.

Intravenous injection: 0.1-0.5 mg/kg, once daily.

Treatment cycle :

Short term: 7-14 days

Long-term: 1-6 months

Evaluation indicators :

Lifespan extension

Behavioral tests

Histological analysis

Telomere length variation

Mechanism of action study :

Telomerase activity assay

DNA methylation analysis

Transcriptome sequencing

Proteomics

Why choose N-acetylepitalonamide made in China?

Technical advantages :

Mastering core modified peptide synthesis technology

Microwave synthesis improves efficiency and quality

Advanced three-dimensional purification system

Complete quality research platform

Quality Guarantee :

Purity ≥ 99.0%

Strict chiral control

Complete activity verification

Batch consistency ≥98%

Application Support :

Provide detailed technical documents

Experimental protocol optimization service

Data analysis support

Custom synthesis service

Cost -effectiveness :

Significant price advantage

Stable and timely supply

Flexible packaging specifications

Technical Support Professional

Research and application directions

Biology of aging :

Telomere maintenance mechanism

Regulation of cellular senescence

Epigenetic aging

stem cell aging

Extended lifespan :

Life extension mechanism

Healthy lifespan research

aging intervention strategies

Anti-aging drug development

Tissue regeneration :

Tissue Engineering

Damage Repair

regenerative medicine

stem cell therapy

Disease model :

Age-related diseases

Degenerative diseases

Cancer and aging

Metabolic aging

Preclinical research data reference

In vitro activity :

Telomerase activity increased 3-5 times

Telomere elongation rate increased by 2-3 times

Cell proliferation capacity increased by 30-50%.

A 40-60% reduction in aging markers

Internal therapeutic effects :

Lifespan extended: 20-30%

Extended healthy lifespan

Telomere length maintenance

Improved organizational function

Pharmacokinetics :

Half-life: 2-3 hours

Bioavailability: ≥85%

Distribution: Widely distributed in various tissues

Metabolism: Peptidase degradation

Security :

Acute toxicity: LD50 > 500 mg/kg

Subacute toxicity: No obvious toxicity

Non-genotoxic

No teratogenicity

Summarize

N-acetylepitalonamide, as a highly efficient telomerase-activating peptide , holds significant value in anti-aging and regenerative medicine research . Chinese manufacturers, through advanced synthetic modification technologies, rigorous quality control, and comprehensive activity validation , provide researchers with high-quality, highly active research tools . The product's superior stability and bioactivity offer strong support for aging biology research and anti-aging drug development.

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