Description

Technical Parameters

GDF -8 (myostatin) 1mg/bottle Core Introduction

Research -grade freeze-dried powder | GDF-8 (Myostatin), a negative regulator of muscle growth

Protein type : Transforming growth factor-β (TGF-β) superfamily member

Molecular weight : approximately 25 kDa (precursor protein)

Active form : 12.5 kDa dimer

Purity : ≥95% (SDS-PAGE)

Specifications : 1mg/bottle

Appearance : White freeze-dried powder

Core Effects

GDF-8 (myostatin) is a negative regulator of muscle growth . Due to its unique muscle mass-limiting mechanism , it is widely used in muscle atrophy research, metabolic disease model construction, and muscle growth regulation mechanism research .

✅ Muscle growth inhibition :

Specifically inhibits myoblast proliferation and differentiation , reducing muscle mass

Activates the Smad2/3 signaling pathway and downregulates muscle-specific gene expression

✅ Pathological model construction :

Simulating muscular dystrophy to study the mechanisms of muscle wasting diseases

Build a metabolic syndrome model to study the interaction between muscle and fat metabolism

✅ Research Tool Value :

Key tool for studying the regulatory mechanisms of muscle growth

Drug screening platform to develop anti-muscle atrophy therapies

Usage Protocol

1. Recombination method :

Fill the lyophilized powder vial with 1 mL of sterile PBS (pH 7.4)

Gently swirl until completely dissolved; avoid vortexing or vigorous shaking

Storage after reconstitution : Refrigerate at 2-8°C and use within 24 hours

2. In vitro research applications :

Cell Culture Treatments :

Concentration : 10-100 ng/mL

Processing time : 24-72 hours

Effect : Inhibits C2C12 myoblast differentiation

3. In vivo research applications :

Animal model construction :

Route of administration : intraperitoneal injection or subcutaneous injection

Dosage : 100-500 μg/kg body weight

Frequency : Once daily for 7-14 consecutive days

Effect : 20-30% reduction in muscle mass

Storage Guidelines

1. Unrestored :

Temperature : -80°C for long-term storage, -20°C for short-term storage

Humidity : Relative humidity ≤ 30%, desiccant protection

Light : Store away from light, in brown glass bottle or wrapped in aluminum foil

Expiration date : 36 months at -80°C, 12 months at -20°C

2. Storage after reconstitution :

Use immediately : Optimal activity is achieved when used immediately after reconstitution.

Store in refrigerator : 2-8°C for no more than 24 hours

Avoid freezing : reconstituted solutions should not be repeatedly frozen and thawed

3. Transport Conditions :

Ultra- low temperature transport : dry ice transport (-78°C)

Shockproof packaging : prevents severe vibrations from affecting protein structure

Fast delivery : Delivery within 48 hours to ensure activity

Why choose GDF-8 ?

Key regulatory factors for muscle growth , mechanism research is indispensable

Construction of multiple disease models , with high translational research value

High-quality recombinant protein with good batch-to-batch consistency

Quality Assurance :

GMP -compliant production , each batch tested for purity by SDS-PAGE (≥95% )

Third-party laboratory report (COA) is included with the product

Endotoxin detection (<1.0EU/mg)

Worldwide Shipping :

Medical vial packaging , rubber stopper seal

Dry ice transportation to ensure protein activity

Private delivery , no logo on the outer packaging

Conclusion

GDF-8 is an essential tool for studying muscle atrophy and growth regulation mechanisms . It is particularly suitable for muscle wasting disease models, muscle metabolism research, and drug screening platforms . The recommended concentration is 10-100 ng/mL (in vitro) and 100-500 μg/kg (in vivo) .

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